Variability in enzyme activities of Trichoderma spp.
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Authors
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SOMESHWAR BHAGAT* SITANSU PAN
Abstract
The effect of different substrates as carbon sources on enzyme activities of Trichoderma spp.in minimum synthetic medium (MSM) was studied. There was marked improvement in the chitinase activities of Trichoderma spp. when the medium was supplemented with either chitin or mycelial powder of M. phaseolina or S. rolfsii, ï¢-1, 3 glucanase, ï¢-1, 4 glucanase, and cellulase activities of Trichoderma spp were also significantly enhanced with addition of glucan, CMC (Carboxymethyl cellulose) and mycelial powder of Pythium aphanidermatum in the basal medium, respectively. The isolate TvAN-3 was most efficient with highest chitinase activity when this isolate was grown in medium supplemented with colloidal chitin (26.5 and 37.0 Pkat/ ml), mycelial powder of M. phaseolina (19.0 and 29.5 Pkat/ml) and S. rolfsii (19.0 and 28.8 Pkat/ml), followed by TvAN-5, ThrAN- 5, ThrAN-7, ThrWB-1 and the isolate ThmAN-10 and ThrWB-2 were the lowest chitinase producers under similar conditions. Highest ï¢-1, 3 and ï¢-1, 4 glucanase activities were expressed with ThrAN-5 (13.6 and 14.9 nkat/ml) and ThrWB-2 being the lowest enzyme activity of only 6.4 and 7.5 nkat/ml. ThmAN-4 was most efficient in secreting cellulase in the culture medium irrespective of other carbon sources. The other best isolates of Trichoderma were ThrAN-5, TvAN-3, TvAN-5, ThmAN-10, ThrAN- 7, ThrWB-1; and TvAN-8 being the least effective isolate in view of their cellulase activities.
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