RAPD-based polymorphism specific to fuscous blight bacterium in grain legume
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Abstract
Three bacterial blights, namely common, fuscous and halo blight are the major biotic threats in grain legumes production. The polymorphism in genomic fingerprints of the three blight bacteria was studied using random primers. Among the primers tested, SBSB02 specifically amplified two fragments of ~300 bp and ~1600 bp in fuscous blight bacterium, but not in common and halo blight bacterium. The polymorphic bands were found in all 8 isolates of X. c. pv. phaseoli var. fuscans, suggesting a high degree of conservation of the amplified regions.
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