Differential inoculation techniques of Colletotrichum acutatum on different part of pepper
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Authors
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K.K. PANDEY*
Abstract
An experiment was conducted to test the differential infectivity of Colletotrichum acutatum by inoculating different plant parts of pepper (Capsicum annum) on six different varieties. The conidial concentration 1x106 per ml from five days old culture was used for artificial inoculation. Inoculation of leaves showed infection as hypersensitive reaction in most of the varieties. The lesion size on twigs varied from 2.0 to 9.6 mm at cent percent infection site. Severity was much more pronounced on vertical inoculated twigs with complete rotting in comparison to horizontal inoculation. On fruits, lesions were more prominent and clear on pinpricked fruits than apical cut fruits. Petal infection started showing symptoms as yellowish brown followed by orange brown with dry rotting. PBC-932-6-6-1 was having maximum resistance against disease in comparison to other varieties. The findings indicate that C. acutatum can infect all above plant parts on challenge inoculation. However, the degree of infectivity depends on differential resistance response of varieties and methods of inoculation. Inoculation on vertical cut end of stem was best technique for creating die-back infection while surface inoculation was best initiating anthracnose infection which can be successfully utilized for quick screening and to study relation between dieback and anthracnose.
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