Detection of DNA A and DNA β associated with cotton leaf curl and some other plant diseases caused by whitefly transmitted geminiviruses
179 / 306
Authors
-
G. RADHAKRISHNAN, V.G. MALATHI and A. VARMA*
Keywords:
Cotton leaf curl disease, geminiviruses, CLCuRV, DNA-β, whitefly, detection, nucleic acid spot hybridization, PCRAbstract
Recent outbreak of cotton leaf curl disease, caused by whitefly transmitted Cotton leaf curl Rajasthan virus (CLCuRV), is a major threat to cotton production in the North-West India. For rapid and sensitive diagnosis of CLCuRV nucleo-diagnostic methods have been developed. Nucleic acid spot hybridization (NASH), using radio-labeled probes to CLCuRV DNA-A and CLCuRV DNA β, was found efficient in detecting CLCuRV both in plants and viruliferous whiteflies. These probes also detected infection by other whitefly transmitted geminiviruses (WTGs) in crop, ornamental and weed plants. Hybridization of DNA β probe with plant samples naturally infected with other WTGs shows that the association of DNA β molecules could be a common feature of WTGs in northern India. CLCuRV was also efficiently detected by PCR using primers to amplify coat protein gene and full length DNA Βmolecules from infected plants and viruliferous whiteflies; subgenomic DNA Βforms were also amplified from CLCuRV infected test hosts and viruliferous whiteflies. The number of sub-genomic forms of DNA β varied in different hosts.
Downloads
Issue
Section
License
For Authors
As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.
For Readers
While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.
All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.