Biochemical responses of tea plants induced by foliar infection with Exobasidium vexans
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Authors
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B.N. CHAKRABORTY, S. DUTTA and U. CHAKRABORTY
Keywords:
Tea, blister blight, enzymes, phenolsAbstract
Biochemical response of tea plants growing in Darjeeling hills exposed to biotic stress due to blister blight infection caused by Exobasidium vexans Massee in the levels of proteins, proline, phenols and enzyme activities such as phenyl alanine ammonia lyase, polyphenol oxidase and peroxidase were studied. Activity of phenylalanine ammonia lyase registered a decline in infected leaves whereas peroxidase and poly phenol oxidase activity was higher. Analysis of peroxidase isozymes by polyacrylamide gel electrophoresis using benzidine as substrate showed four isozymes in healthy and five in infected leaves. One of the most significant changes was observed in proline content which increased markedly in blister infected leaves. Protein showed a decline at very early stages of infection. SDS-PAGE analysis revealed the loss of several proteins following infection. Total phenols were significantly higher in the infected leaves in comparison to the healthy ones. Antifungal activity of phenols from both healthy and blister infected leaves were detected following agar cup bioassay. Antifungal activity was higher in healthy leaves. HPLC analysis of catechin extract from healthy and infected leaves showed little qualitative difference but, some quantitative changes.
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