Pathogenic and biochemical variations in Neovossia indica
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Authors
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S.K. SHARMA, DV SINGH, RASHMI AGGARWAL and K.D. SRIVASTAVA
Keywords:
Wheat, Karnal bunt, Neovossia indica, variability, pathogenic, biochemicalAbstract
Host-pathogen responses on 10 differential hosts distinguished populations of six isolates into three pathotypes, pathotype I (KB-1 ,-2 and -4), pathotype II (KB-3 and -5) and pathotype III (KB-6). Isolate KB2, which was the most virulent, showed the highest content of lipid (131.1 mg/g), nitrogen (24 mg/g), protein (15.3%), sugars (35 mg/g) and reducing sugar (0.131 mg/g). Comparatively, KB-6 which was less aggressive and slower growing isolate, had the minimum amount of these constituents. KB-2 could be distinguished from other isolates by the presence of linolenic acid and KB-6 by the absence of capric and pentadecenoic acids. Polyacrylamide gel electrophoretic analysis reflected variations in soluble protein fractions among the three differently virulent pathotypes of N. Indica. In KB-6, 23 bands were resolved, of which 16 were common to all the representative isolates. Esterase isozyme detected in the mycelial extracts revealed the presence of four bands of different Rf values in KB-6. However, isolates KB-2 and KB-5 lacked bands with an Rf value of 0.76. Analysis of peroxidase isozyme showed the presence of six bands in KB-2 and KB-5, but in KB-6 a band of 0.87 Rf was missing, confirming variation within the isolates.
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