Molecular characterisation of isolates of the sugarcane red rot pathogen
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Authors
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D. MOHANRAJ, S. KUMARESAN and TV SREENIVASAN
Keywords:
Sugarcane red rot, Colletotrichum falcatum, isolate characterisation, RAPDAbstract
Isolates of the sugarcane red rot pathogen (Colletotrichum fa/catum) showing variations in their pathogenicity on host cultivars were compared for their genetic relationship using RAPD studies. DNA isolated from 7 major pathotypes of the organism was amplified using PCR procedures with 80 random primers. Of these 61 primers exhibited well defined DNA polymorphism among the pathotypes. Data from all polymorphic primers were pooled and similarity index calculated. Roger's genetic distance co-efficients ~ were computed for all pairwise combinations and a dendrogram developed to represent the genetic relatedness of the isolates. The isolate CO 7717 recorded the highest genetic distance from the other pathotypes with a co-efficient value of 0.291 and it showed maximum distance from the isolate CO 1148. The other isolates expressed different levels of relatedness among themselves. The grouping of the isolates based on RAPDs was in general agreement with their pathogenicity on different host clones. Implications of the findings on screening for red rot resistance and cultivar deployment are discussed.
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