Bi-directional PCR- a tool for identifying strains of Citrus tristeza virus
219 / 173
Authors
-
AVIJIT ROY and P. RAMACHANDRAN
Keywords:
Bi-directional polymerase chain reaction, BO/PCR, CTVAbstract
A technique developed based on sequence of the coat protein of Citrus tristeza virus (CTV) was used to amplify the CP gene by RT-PCR. The amplified product was detected by agarose gel electrophoresis which showed a band of -700 bp in CTV infected samples but not in healthy. Using different primer combination, it was shown that CTV isolates, tested from four locations in the country, belong to the severe strain. The bi-directional PCR technique has been used for the first time in the country for detection of CTV strains. Its usefulness under Indian conditions is discussed.
Downloads
Issue
Section
License
For Authors
As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.
For Readers
While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.
All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.