Isozyme variability among isolates of Ascochyta rabiei

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  • G. UMA DEVI, MAHENDRA PAL and NEENA MIITER Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110 012


Ascochyta rabiei, variability, isozymes, cluster analysis


Positive activity was observed for 7 out of 12 isozymes tested in native polyacrylamide gel electrophoresis (PAGE) for five isolates of Ascochyta rabiei viz., 011, G2, GL 866, ICH and ICK differing in pathogenicity. Esterase, malate dehydrogenase, acid phosphatase and catalase gave a polymorphic banding pattern whereas peroxidase, alkaline phosphatase and glutamate dehydrogenase resulted in monomorphic banding pattern. Identical protein profile for all the isolates with respect to major bands and differences in the minor bands were observed in SOS-PAGE for total proteins. UPGMA method of cluster analysis grouped these isolates into two new clusters with 011 and G2 in one cluster and GL 866, ICH and ICK in the other cluster. In the second cluster, isolate ICH was further separated from GL 866 and ICK.




Research Articles

How to Cite

Isozyme variability among isolates of Ascochyta rabiei. (2002). Indian Phytopathology, 54(1), 16-22.