An in vitro screening technique for the identification of grain mould resistance in sorghum
Abstract views: 159
/ 
PDF downloads: 95
Authors
-
S.D. SINGH and S.S. NAVI
Genetic Resources and Enhancement Program. International Crops Research Institute for the Semi-Arid Tropics.
Patancheru 502 324
Keywords:
Grain mould, Fusarium sp., Curvularia sp., screening techniqueAbstract
Grain mould (GM) is the most important biotic constraint of sorghum [Sorghum bieolor (L.) Moench] in India and other parts of the world and host plant resistance is the most convenient method o( control of this disease. Breeding for disease resistance has been slow due to the total reliance on field screening which is used once-a-year during rainy season. In order to conduct screening independent of season and to detect possible escapes from field screening, an in vitro screening technique has been developed. The technique involves dip inoculation of mature seed in a mixed spore suspension (lx106 spores ml') of major GM fungi (Fusarium moniliforme, F. pallidoroseum, and Curvularia lunata), transferring inoculated seed to the pre-sterilized petridish humid chamber @ 25 seed plate+, and their incubation at 28±1°C with or without light for 5 days. Visual evaluation of mould infection was done on 1-9 scale (1 = no colonization, and 9 = >75% seed surface colonization by the mould fungi) using an illuminated magnifier. The efficiency of the technique was measured by comparing mean grain mould scores (MGMS) of 43 accessions evaluated at Bhavanisagar, Mysore and Patancheru in India. There was a significant correlation (P<O.Ol) between ill vitro MGMS and field scores at or across three locations. Of the 66 photoperiod sensitive accessions that were screened using the in vitro screening, 14 showed high level of resistance even under field disease nursery situation. The usefulness of the technique in GM resistance program is discussed.
Downloads
Issue
Section
License
For Authors
As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.
For Readers
While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.
All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.