Differentiation of biologically distinct Cucumber mosaic isolates by double stranded RNA profile
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Authors
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SHELLY PRAVEEN, SAVARNI TRlPATHI and B.K. GIRl
Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012
Keywords:
CMV, ds-RNA, tobaccoAbstract
Four isolates of Cucumber mosaic virus from Nicotiana tabacum, Commellina species and Petunia, designated as MV- T, C, PI and P 2 , differed in their host reaction, temperature sensitivity, and at the molecular level. Serologically related isolates of CMV viz. CMV-P I , P 2 , C and T were analyzed on the basis of their symptomology, temperature tolerance and double stranded RNA pattern. Double stranded RNA (ds-RNA) pattern of these CMV isolates representing biological diversity were examined. Two distinct patterns differing by the number and or/position of the ds- RNA bands were obtained for the four isolates. The ds-RNA pattern can not be necessarily co-related with the symptoms severity nor with any specific biological characteristics, however, it can be utilized in distinguishing isolates.
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