Studies on development of Trichoderma viride mutants and their effect on Ustilago segetum tritici
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Authors
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R. SELVAKUMAR, K.D. SRIVASTAVA, RASHMI AGGARWAL, D.V. SINGH and PREM DUREJA
Division of Planl Pathology, 1 Division of Agricultural Chemicals, Indian Agricultural Research Institute,
New Delhi IJO OJ2
Keywords:
Ustilago segetum tritici, biocontrol, Trichoderma viride, carboxin tolerance, mutationAbstract
Carboxin tolerance in Trichoderma viride was induced by exposing the culture to UV light and EMS (ethyl methane sulfonate). Three stable mutants showed different colony characters, fungicide tolerance and antagonistic potential against Ustilago segetum var. tritici from the parent strain. One of the strains, TV5-2, showed better antagonistic potential, reducing chlamydospore germination to 14.1% and mycelial dry weight of the pathogen to 41.5 mg compared with 94.1% and 87.5 mg in the respective controls. The strain could tolerate up to 50 鎔/ml of carboxin and produce more conidia on PDA.
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