Role of lytic enzymes in yellow mould disease of Agaricus bisporus
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Authors
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SHWET KAMAL, R.N. VERMA and H.S.S. SHARMA
National Research Centre for Mushroom. Chambaghat, Solan 173 213
Keywords:
Agaricus basporus, lytic enzyme, yellow mould diseaseAbstract
Role of lytic enzymes produced by yellow mould causing organisms, viz. Myceliophthora lutea, Sepedonium chrysospermum [Apiocrea chrysosperma] and S. maheshwarianum, in the pathogenesis and death of mushroom mycelium have been investigated and results so far obtained are reported. Interactions seen in nutrient poor medium exhibited coiling and penetration of M. lutea hyphae into Agaricus bisporus hyphae while antagonism and necrosis were observed in case of Apiocrea chrysosperma. No such symptoms were recorded in the case of S. maheshwarianum. Lytic effect of 1-3,beta-exoglucanase and 1-3(4),beta-endoglucanase [cellulase] enzymes on Agaricus bisporus (S-11) mycelia was studied. Microscopic examination of these mycelia after staining with 1% rose Bengal revealed unstained hyaline portion in the hyphae, possibly due to the loss of cell wall and cell constituents caused by lysis of the hyphae. Such clear zones were maximum in mycelia dipped in culture filtrate of M. lutea, while S. maheshwarianum didnot produce such clear zones. Apiocrea chrysosperma, however, could cause some lysis of Agaricus bisporus mycelium.
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