Comparison of three serological assays for the detection of potyviruses
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Authors
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A.I. BHAT*, A. VARMA and R.K. JAIN
Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute,
New Delhi 110 012
Keywords:
Potyvirus, detection, sensitivity, serology, Ffab'), ELISA, dot immunobinding assay (DIBA), electro-blot immunoassay (EBJA)Abstract
The sensitivity of three serological assays, indirect F(ab')2-ELISA, dot immunobinding assay (DIBA) and electroblot immunoassay (EBIA), in detecting cowpea aphid-borne mosaic, henbane mosaic and potato virus Y viruses was compared. DIBA was the mostsensitive assay, detecting upto 100 pg of virus followed by F(ab')2-ELISA which could detect upto 1 ng of virus in purified preparations diluted with sap extracts from respective non-infected tissues. EBIA could detect upto 100 ng of the virus.
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