Characterization of cucumber mosaic cucumovirus isolates naturally infecting three solanaceous vegetable crops in Andhra Pradesh
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Authors
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G. KIRANMAI, P. SREENIVASULU* and M.V. NAYUDU
Department of Virology, Sri Venkateswara University, Tirupati 517 502
Keywords:
Tomato, chilli, brinjal, cucumber mosaic cucumovirus isolates, characterizationAbstract
Viruses isolated from commercial tomato, chilli and brinjal crops in Chittoor district, Andhra Pradesh were identified as strains of cucumber mosaic cucumovirus (CMV) on the basis of host range, transmission, serology and physicochemical properties. Dolichos lablab for CMV-tomato isolate, Cucumis sativus for CMV-brinjal isolate and Datura metel for CMV-chilli isolate were identified as differential diagnostic host plants. The longevity in vitro was 4-6 days, the thermal inactivation point was 50-65°C and the dilution end point 10.3-10" for the three isolates. Myzus persicae tansmitted the three isolates in a nonpersistent manner. The titres of polyclonal antisera produced against three isolates ranged from 1:5000 to 1:23000 in DAC-ELISA. CMV-brinjal and CMV-chilli isolates were serologically different but related to CMV-Tomato isolate. The Mr of coat protein of three isolates was ranged from 24.5 to 25.7 x 1Q3 d. The Mr of three genomic RNA sand one subgenomic RNA of three isolates was 1.24 to 1.26; 1.05 to 1.12; 0.78 to 0.86 and 0.34 to 0.39 x 106 d. The dsRNA isolated from infected chilli, tomato and brinjal was resolved into three species with Mr ranged from 2.48 to 2.52; 2.10 to 2.26 and 1.56 to 1.72 x 106 d. The tomato, chilli and brinjal isolates of CMV were identified as CMV-To, CMV-Ch and CMV-Br strains, respectively.
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