Use of a molecular tracking system to study the survival and migration of Pseudomonas putida, a biocontrol agent for sheath blight disease of rice
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S. MAGESWARI and S.S. GNANAMANICKAM
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Madras 600 025
Keywords:
lacZY genes were inserted into P. putida Vl4i which is a biocontrol agent for sheath blight disease of rice caused by Rhizoctonia solani. The marked strain, P. putida Vl4i-gal had blue coloured colonies which aided in easy detection of the presence ofAbstract
The lacZY genes were inserted into P. putida Vl4i which is a biocontrol agent for sheath blight disease of rice caused by Rhizoctonia solani. The marked strain, P. putida Vl4i-gal had blue coloured colonies which aided in easy detection of the presence of the bacterium in rice seed, root or leaf tissues. In a greenhouse experiment it was observed that the marked strain persisted in IR50 rice roots for 60 d in sterile soil or for 50 d in nonsterile soil after the seeds were coated with the bacterium. It migrated to rice seedlings only for 10 d after seedling emergence. The bacterium survived in rice foliage for 40 d after it was applied as a foliar spray application. The wild-type and the marked P. putida Vl4i strains afforded 61-62% sheath blight suppression in IR50 rice.
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