Isolation and characterization of a strain of African cassava mosaic virus from a local cassava in the Republic of Benin
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Authors
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AHOHUENDO, B.C.1 and S. SARKAR2
1 Faculte des Sciences Agronomiques/Universite Nationale du Benin, B.P. 526 Cotonou, Republique du Benin
2 lnstitut fur Phytomedizin, Universitat Hohenheim, 70593 Stuttgart, Germany
Keywords:
African cassava mosaic, geminivirus, capsid protein, cytopathic effects, ELISAAbstract
Virus particles were purified from infected local cassava and the virus was characterized. Virus particles measured approx. 36X23 nm. Purified virions or their nucleic acid transmitted to Nicotiana benthamiana, N. debneyi, N. clevelandii, and Daturastramonium plants showed a difference in symptom expression compared with another strain of the virus received from the German Collection of Microorganisms in Braunschweig. Viral capsid protein was resolved by SDS-PAGE. Its estimated MW was 30 kDa. A single nucleic acid of estimated MW of 0.7X106 Da was isolated from virus particles and resolved by agarose gel electrophoresis. Thin-section electron microscopy of infected and healthy leaf of cassava and of N. benthamiana plants revealed no cytopathic effects common to whitefly-transmitted geminiviruses. Ultrastructural features like hypertrophy and segregation of nuclear components were observed in ACMV-infected leaves of cassava and of N. benthamiana plants, but they could also be rarely detected in healthy materials. With antisera prepared to ACMV, the virus content in different parts of cassava plants were determined by ELISA. The virus isolated from the local cassava and the strain received from Braunschweig were identified with the monoclonal antibody SCR 23 as strains of the geminivirus group A (strains of West-Africa and West-Kenya).
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