FACTORS INFLUENCING THE SEDIMENTATION PATTERN OF RICE TUNGRO VIRUS (RTV) COMPONENTS DURING DENSITY-GRADIENT CENTRIFUGATION
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Authors
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R. K. JAIN AND M. D. MISHRA
Division of Mycology and Plant Pathology
Indian Agricultural Research Institute, New Delhi 110 012
Keywords:
Tungro, RTV components, Sedimentation pattern, RiceAbstract
Optimal density-gradient conditions necessary for better resolution of RTV components during purification were identified. Both RTSV and RTBV particles sedimented differentially, when centrifuged at 28,000 rpm for 1/2, 1 and 2h. The two particles were .best resolved to fractions 5 and 6 (RTSV), and 11 (RTBV), when the virus preparation was centrifuged at the centrifugal effect of 0.031 x 1013 radians squared per second (28,000 rpm, 1h) through 10·40 per cent sucrose gradients prepared in EDTA buffer (pH 8, 0.01 M). Virus titre and heat treatment of the loading sample were found to affect the resolution and the two particles were completely denatured at 60°C. Storage of virus preparation upto 30 days at 4°C did not influence the resolution of the two particles.
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