ISOLATION AND PURIFICATION OF A VIRUS INFECTING PIPER BETLE
Abstract views: 65
Authors
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J. K. JOHRI, K. M. SRIVASTAVA, R. K. RAIZADA, A. L. DESHPANDE* AND B. P. SINGH
National Botanical Research Institute, Lucknow 226 001
Keywords:
Piper betle, Flexuous rod shaped virus, Crystalline inclusionsAbstract
Betelvine leaves showed yellow spots which, at acute stages, became completely yellow. The virus was graft transmissible but not by mechanical means. Purified virus showed flexuous rods of 730 x 12 nrn which had a buoyant density of 1.27 glcm3 in cesium sulphate. Molecular weight of the protein subunits was determined as 32 KD. Crystalline cytoplasmic inclusion bodies were observed in infected cells.
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