RAPD based DNA fingerprinting of toxigenic and non-toxigenic strains of Aspergillus flavus isolated from different habitats
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Authors
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AJOY KUMAR CHOUDHARY*, PRIYANKA KUMARI and RASHMI AGGARWAL
Keywords:
Aflatoxin, Aspergillus flavus, cluster analysis, PCRAbstract
Aflatoxins produced by Aspergillus flavus are extremely toxic substances to man and animals. These fungi are ubiquitous which grow on a wide variety of substrates. Isolations made from different habitats have shown that not all the strains of A. flavus are toxigenic. DNA polymorphism of 16 toxigenic and non-toxigenic strains of A. flavus isolated from different habitats (air, soil, maize) studied through RAPD technique, grouped the isolates into different clusters based on similarity matrix and UPGMA method. These results support the need of using molecular marker as an auxillary tool in differentiating different strains of A. flavus.
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