Identification and validation of leaf rust resistance genes in Indian wheat genotypes using molecular markers


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Authors

  • MALKHAN SINGH GURJAR*, RASHMI AGGARWAL, SAGAR BANERJEE, T.K. BAG and MOHINDER PRASHAR

Keywords:

DNA markers, Lr genes, Puccinia triticina, Triticum aestivum

Abstract

A collection of 25 wheat cultivars/genotypes were evaluated for leaf rust resistance genes using different pathotypes and presence of resistance genes was confirmed through molecular markers. The presence of different resistance genes like Lr1, Lr10, Lr19, Lr26 and Lr34 were identified through gene matching technique. Different Lr genes were further confirmed using five different PCR-based markers such as STS and SCAR markers linked to leaf rust resistance genes. The presence of Lr1, Lr10, Lr19, Lr26 and Lr34 in the tested genotypes was confirmed by a unique amplification of markers pTAG621560, Lrk10D1282,362, SCS265512, IB 267267 and csLV34F229,150. Of 25 genotypes, the presence of Lr1 gene was found in all wheat genotypes except HD 2888 and MACS 6145. Genotypes, HD 2985, VL 804, Raj 3765, HI 977, PBW 343, HS 240 and NI 5439 showed the presence of 282 bp fragment specific to Lr10 gene. A single fragment of 512 bp (SCS265) specific to Lr19 did not give amplification in all wheat genotypes. A 267 bp fragment with marker (IB-267) got amplified in genotypes, HD 2733, HD 2824, VL 804, UP 2425, DBW 17, PBW 343, PBW 550, HS 240 and MACS 1967 carrying Lr26 gene. Six genotypes namely HD 2985, VL 616, UP 2425, Raj 3765, DBW 16 and PBW 550 showed the presence of 150 bp fragment (cs LV34) specific to Lr34 gene.

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Issue

Vol. 67 No. 4 (2014): Indian Phytopathology

Section

Research Articles

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How to Cite

SAGAR BANERJEE, T.K. BAG and MOHINDER PRASHAR, M. S. G. R. A. (2014). Identification and validation of leaf rust resistance genes in Indian wheat genotypes using molecular markers. Indian Phytopathology, 67(4), 358-364. https://epubs.icar.org.in/index.php/IPPJ/article/view/45494