Secondary metabolite profiling of plant pathogenic Alternaria species by matrix assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry


260 / 456

Authors

  • M. JYOTHI LAKSHMI, P. CHOWDAPPA* and RIAZ MAHMOOD

Keywords:

Alternaria, HPLC, MALDI–TOF MS, secondary metabolites, TLC

Abstract

Profiling of secondary metabolite production (both known and unknown metabolites) on standardized culture media has proven to be useful for classification and identification of certain morphologically similar species of Alternaria. In this study, secondary metabolite profiling of 50 fungal isolates belonging to 10 plant pathogenic Alternaria species such as A. solani, A. porri, A. brassicicola, A. brassicae, A. sesame, A. alternata, A. macrospora, A. ricini, A. carthami and A. brunsii isolated from vegetable, oil yielding and seed spice crops were examined. Secondary metabolites were extracted from 14 day old cultures, grown on potato dextrose agar, with ethyl acetate containing formic acid. After extraction, the secondary metabolite profiles of all fungal isolates were analyzed using thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and matrix assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry. These analyses indicated characteristic ‘species-specific metabolite finger prints’. Thus, chemotaxonomic approach is a simple and rapid technique to determine the chemical diversity of the different Alternaria species and to identify species specific metabolites that could be adopted as chemotaxonomic markers in species identification. This study can be integrated in a polyphasic approach.

Downloads

Issue

Vol. 67 No. 4 (2014): Indian Phytopathology

Section

Research Articles

License

For Authors

As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.

For Readers

While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.

How to Cite

and RIAZ MAHMOOD, M. J. L. P. C. (2014). Secondary metabolite profiling of plant pathogenic Alternaria species by matrix assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry. Indian Phytopathology, 67(4), 374-382. https://epubs.icar.org.in/index.php/IPPJ/article/view/45500