Isolation technique and culture conditions of false smut pathogen (Ustilaginoidea virens) on rice


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Authors

  • MATHEW S. BAITE* and R.K. SHARMA

Keywords:

Cultural characters, false smut, isolation technique, ITS sequencing, rice, U. virens

Abstract

False smut disease is caused by Ustilaginoidea virens (Cooke) Takahashi on rice. It has become a serious pathogen in almost all rice-growing areas in the world. The conditions for successful isolation of pathogen in axenic cultures have been standardized and the growth conditions were optimized. The fastest growth rate was achieved with potato sucrose agar medium having a pH of 6.0 at an incubation temperature of 27°C. Dark incubation was also found highly conducive for the growth of fungus compared to incubation in light. These conditions could be useful for the best isolation of the pathogen for different studies. Beside morphological identification, the identity of the fungal pathogen was confirmed through ITS sequencing which showed up to 98 % identity with U. virens in NCBI-BLAST analysis. The proposed isolation technique coupled with information on optimization of culture conditions of U. virens will immensely contribute to further studies on this fungus and the disease in India.

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Issue

Vol. 68 No. 1 (2015): Indian Phytopathology

Section

Research Articles

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How to Cite

R.K. SHARMA, M. S. B. and. (2015). Isolation technique and culture conditions of false smut pathogen (Ustilaginoidea virens) on rice. Indian Phytopathology, 68(1), 50-55. https://epubs.icar.org.in/index.php/IPPJ/article/view/46972