Identification of Bipolaris sorokiniana-responsive differential transcripts in wheat (Triticum aestivum L.)
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Authors
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RASHMI AGGARWAL
Division of Plant Pathology,
Indian Agricultural Research Institute,
New Delhi 110 012
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LALIT L. KHAREBIKAR
Division of Crop Improvement,
Central Research Institute for Jute and Allied Fibers (ICAR),
Barrackpore, Kolkata 700 120
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AND RENU
Haryana State Council for Science and Technology,
Sector 2, Panchkula 134 151
Abstract
Differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) is used to identify transcripts induced by Bipolaris sorokiniana infection in wheat (Triticum aestivum L.). Using this method, we have identified two mRNA transcripts that are up- or down-regulated following B. sorokiniana infection. With 12 primer combinations, a total of 93 differential display products were observed in resistant genotype, BH1146 compared to 89 differentially displayed products in susceptible genotype, Sonalika. These products demonstrated qualitative and quantitative differences in the expression. The differentially expressed transcripts were collectively named WTRB (Wheat Transcripts Responsive to Bipolaris). We have identified two WTRBs, which were significantly altered due to B. sorokiniana infection. Both WTRB-1 and WTRB-2 expressed for longer period in resistant line compared to the susceptible line and may be used as molecular markers for screening wheat lines for spot blotch resistance.
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