Intraspecific variation in Tilletia indica, a pathogen causing Karnal bunt of Wheat


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Authors

  • MALKHAN SINGH GURJAR*, ABHIMANYU JOGAWAT DEEPIKA KULSHRESHTHA, SAPNA SHARMA ROBIN GOGOI and RASHMI AGGARWAL

Keywords:

Genetic diversity, Karnal bunt (KB), partial Gapdhgene, sequencing, Tilletia indica

Abstract

in the present climatic change conditions. A total of twenty one Karnal bunt (KB) diseased samples were collected from North western plain zone of wheat in India during 2013 to 2015. The KB coefficient of infection was varied minimum of 1.25 percent and highest of 14.25 per cent in Bundi, Rajasthan and Aligarh, Uttar Pradesh respectively. The partial Gapdh gene was amplified and gave unique 694 bp fragments in all isolates of T. indica and then sequenced. All KB isolates clustered in two major clades of phylogeny tree. Seventeen isolates viz. KB1, KB7, KB11, KB15, KB12, KB16, KB19, KB3, KB2, KB10, KB9, KB17, KB20, KB18, KB2, KB6, KB13 clustered in clade I and four isolates viz. KB5, KB4, KB8, KB14 clustered in clade II with outlier of Tilletia horrida and Ustilago maydis. Among isolates, five isolates KB1 (Bareilly, Uttar Pradesh), KB12 (Bulandshar, Uttar Pradesh), KB7 (Aligarh, Uttar Pradesh), KB11 (Bulandshar, Uttar Pradesh) and KB15 (Kota, Rajasthan) matched 100 per cent in subgroup I of clade I and two isolates KB18, KB21 were also similar in subgroup II of clade I. Majority of T. indica isolates did not cluster to region wise and showed nucleotide variation which ranged from one to two. This study indicated that T. indica isolates showed the high genetic variation at DNA level in single KB seed containing mass of teliospores and can be helpful to understand the pathogen behaviour.

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Submitted

2016-12-22

Published

2016-12-24

Issue

Vol. 69 No. 4 (2016): Indian Phytopathology

Section

Research Articles

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How to Cite

DEEPIKA KULSHRESHTHA, SAPNA SHARMA ROBIN GOGOI and RASHMI AGGARWAL, M. S. G. A. J. (2016). Intraspecific variation in Tilletia indica, a pathogen causing Karnal bunt of Wheat. Indian Phytopathology, 69(4), 352-356. https://epubs.icar.org.in/index.php/IPPJ/article/view/66242