Virulence and genetic diversity of Puccinia striiformis f. sp. tritici isolates in Punjab, India
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Keywords:Diversity, pathotype, Puccinia striiformis, stripe rust, wheat
AbstractVirulence diversity was determined among 16 isolates of Puccinia striiformis f. sp. tritici was analyzed by inoculating isolates collected from diverse areas of Punjab on differential lines carrying known resistance genes. Pathotypes 78S84, 46S119 and 110S119 obtained from IIWBR, Regional Station, Flowerdale, Shimla were also included as check. All the Pst isolates were virulent on lines carrying genes Yr2, Yr3, Yr4, Yr6, Yr7, Yr8, Yr9 and were avirulent on genes Yr1, Yr5 and Yr10. Variation for virulence was recorded among isolates on lines having genes YrSD, YrSU and Yr9+. Genetic diversity of the isolates was studied using EST-derived SSR markers. Cluster analysis based on UPGMA algorithm and principal coordinate analysis (PCoA) classified these 19 isolates into three separate clusters which indicated genetic diversity among the isolates from Punjab.
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