Enhancing sporulation and determination of virulence of Alternaria solani isolates infecting tomato


Abstract views: 375

Authors

  • NISHANT PRAKASH* and KARUNA VISHUNAVAT

Keywords:

Alternaria solani, growth media, CaCO3, alternate light and dark

Abstract

In order to study the effect of media, incubation condition and calcium carbonate (CaCO3) amendment on mycelial growth and sporulation in Alternaria solani, 13 different media were examined under different incubation conditions. Out of these, potato dextrose agar, phytone agar and Czapeck’s Dox agar supported maximum mycelial growth at 14 days after incubation at 25±1°C. Incubation condition T2 (One week continuous fluorescent light at 25±1°C followed by one week period dark at 21±1°C) supported sporulation in all isolates of A. solani. Moreover, pearl millet-CaCO3 agar media supported sporulation in all A. solani isolates and found to be the best medium supporting sporulation in A. solani. However, all the CaCO3 supplemented media (HPDA + CaCO3, CaCO3 agar and pearl millet - CaCO3) supported sporulation in A. solani. We further grew a random isolate As-4 under two growth condition viz., (i) subcultured on pearl millet + CaCO3 media and incubated under T2 incubation condition-denoted as isolate As-P and (ii) subcultured on PDA and incubated for 14 days under alternate light and dark condition at 25°C-designated as isolate As-Q. Effect of these growth conditions on virulence of A. solani were evaluated by inoculating both the isolate As-P and As-Q on different tomato germplasm/cultivars under glasshouse condition. Disease severity recorded on tomato germplasm indicated that EC-519795 and Pant bahar were highly susceptible and moderately susceptible to As-P and As-Q respectively. Germplasm Shalimar 2, Palam Pink and Arka Saurabh showed susceptible reaction against As-P while moderately susceptible reaction against As-Q. Disease reaction shown by Roma, Arka Vikas, EC-519770, Palam Pride and Arka Samrat appeared same against both the isolates. Results of glasshouse experiment indicated that A.solani isolate As-P, grown on pearl+CaCO3 under T2 incubation condition appeared more virulence than isolate As-Q, grown on PDA.

Submitted

2018-03-13

Published

2018-03-14

Issue

Vol. 70 No. 4 (2017): Indian Phytopathology

Section

Research Articles

License

For Authors

As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.

For Readers

While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.

How to Cite

KARUNA VISHUNAVAT, N. P. and. (2018). Enhancing sporulation and determination of virulence of Alternaria solani isolates infecting tomato. Indian Phytopathology, 70(4), 471-477. https://epubs.icar.org.in/index.php/IPPJ/article/view/76880