Biocontrol of aflaroot and dry root rot of groundnut by transgenic Rhizobium
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Authors
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AVINASH B. ADE L.V. GANGAWANE*
Abstract
Aflaroot (Aspergillus flavus) and dry root rot (Aspergillus niger) are known to cause considerable loss of groundnut (Arachis hypogea L.). Rhizobial isolates were obtained from the nodules of groundnut and wild legumes such as Indigofera linifolia, I. duthiei, Alysicarpus tetraganolobus and Tephrotia pumila. Pseudomonas fluorescens was isolated from rhizosphere of groundnut and noted to be antagonistic to A. flavus and A. niger. This was also confirmed by plasmid analysis. The horizontal gene transfer from P. fluorescens to Rhizobium was obtained. The transconjugants were used for biocontrol of aflaroot and dry root rot. The percentage control efficacy (PCE) was better due to application of transconjugants.
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