Effect of endophytic bacterial antagonists against black rot disease of cauliflower caused by Xanthomonas campestris pv. campestris
345 / 239
Authors
-
DINESH SINGH*, SHRI DHAR, D.K. YADAV
Abstract
Black rot of cauliflower caused by Xanthomonas campestris pv. campestris is very destructive disease world wide and cause great losses to the crop. Antagonistic activity was tested on nutrient sucrose agar medium in vitro by using two bioagents such as Pseudomonas fluorescens strain PF – 1 and Bacillus subtilis strain BS – 7 against X. campestris pv. campestris. The bioagents were diluted 0, 10, 100 &1000 fold and X. campestris pv. campestris diluted 0, 10, 100, 1000 & 10000 fold for testing bio- efficacy. Maximum inhibition zone (2.4 cm diameter) was formed when P. fluorescens strain PF -1 containing 4.3 × 1010 cfu/ ml and X. campestris pv. campestrs diluted 10000 fold. B. subtilis formed inhibition zone 3.0 cm diameter when its population was maximum (6.4 × 1010 cfu/ ml) and X. campestris pv. campestrs diluted 10000 fold. The inhibition zone was increased with diminishing the population of X. campestris pv. campestrs by using both the antagonists. In foliar application of bioagents and antibiotics, five treatments viz., PF- 1, BS- 7, PF- 1 + BS- 7, streptomycine sulphate (Str) – 250 ppm, and control (sprayed only X. campestris pv. campestrs) were applied on 45 days old plants of cauliflower variety Pusa Snowball at three different times such as bioagent applied one week before pathogen inoculation, bioagent inoculated simultaneously before pathogen inoculation and bioagent inoculated after 3 days of pathogen inoculation. Minimum disease incidence (37.6%) and severity (1.99) found in PF- 1 + BS – 7 after 30 days of inoculation and at par to the antibiotic streptomycin sulphate 250 ppm application when it was inoculated one week before and simultaneously before pathogen inoculation. Combinationof both the bioagents may be applied for better management of black rot diseases.
Downloads
Issue
Section
License
For Authors
As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.
For Readers
While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.
All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.