Morphological, physiological, pathogenic and molecular variability among isolates of Alternaria solani from tomato
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Authors
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M.K. NAIK*, Y. PRASAD, K.V. BHAT, G.S. DEVIKA RANI
Abstract
Morphological, physiological, pathogenic and molecular characteristics of Alternaria solani isolates from tomato were studied for variability. The conidia varied in length, breadth, beak size, septation and in hyphal width. Maximum growth and sporulation was recorded in Sabouraud’s agar followed by PDA supplemented with CaCO3 by ASB2 isolate. Temperature of 250C was optimum with ASB2 and ASG3 recording higher growth. ASG3 produced higher growth and sporulation at 100%RH whereas ASB2 sporulated even at 65% RH. Maximum growth and sporulation was obtained at 12h of alternate light and darkness followed by continuous darkness. The pathogenic reaction indicated ASB2 as more virulent than others. The RAPDPCR analysis indicated maximum similarity (73.78%) in the isolates of Northern Karnataka region (ASR5, ASD4 and ASG3). The isolates ASB2 from Southern Karnataka shared only 45% similarity indicating distinct polymorphism, which was also hyper virulent as indicated in pathogenic variability test.
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