Genetic variability within Fusarium solani as revealed by PCR- fingerprinting based on ISSR markers
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MOHD. ARIF, N.W. ZAIDI, QAZI MOHD. RIZWANUL HAQ and U.S. SINGH*
Abstract
DNA based molecular marker, Inter Simple Sequence Repeats (ISSRs) were used for the study of DNA polymorphism in Fusarium solani associated with shisham wilt as no information is available on variability in F. solani isolates from disease affected plants. Isolation and analysis of genetic diversity were done among 22 isolates of F. solani collected from wilted tissues using molecular marker technology. A total of 10, out of 25 ISSR primers were selected. Amplification of genomic DNA of 22 isolates, using ISSR primers, yielded 98 fragments that could be scored, of which 96 were polymorphic, with an average of 9.6 polymorphic fragments per primer. Number of amplified fragments ranged from 7 (LC-51) to thirteen (LC-65) and varied in size from 325 bp to 3000 bp. Percentage polymorphism ranged from 90 % (LC-64) to a maximum of 100% (with all eight except LC-59 and 64 ), with an average of 97.96%. The cophenetic correlation between two matrices gave r = 0.9804, showing very good fit correlation for a cluster analysis. The similarity coefficient ranged from 0.20 to 0.97. Based on DNA fingerprints 22 isolates were categorized into four clusters. Isolate Fs-4 and Fs-5 showed highest degree of similarity (97%) with each other.
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