Characterization of virus from pumpkin as an isolate of PRSV-W
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Authors
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CHINMAY BISWAS and ANUPAM VARMA
Keywords:
WMV, PRSV-W, Cucurbits, PotyvirusAbstract
Host range of WMV-A was restricted to the family cucurbitaceae and it could produce symptoms on Luffa sp. WMV-A was transmitted through sap and by aphid vectors; but no seed transmission was recorded. Aphis craccivora was found to be a more efficient vector than Myzus persicae in transmitting WMV- AWMV-A was found to produce amorphous cytoplasmic inclusions in the infected leaves of pumpkin and the aggregation of chloroplasts was also observed. WMV-A had a thermal inactivation point (TIP) of 50-55 °C and dilution end point (OOP) between 10-3 and 10-4. It retained infectivity for 24 hours at room temperature (30- 35 °C) and 7 days at 10 °C. The particles of WMV-A were flexuous filaments, typical of potyviruses. The most common size of the particles was found to be 812 x 12 nm. WMV-A showed strong positive reaction with the antiserum of WMV-I. On the basis of various properties the present isolate (WMV-A) was identified to be an isolate of PRSV-W.
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