Detection of brinjal little leaf phytoplasma in phloem elementsisolated by enzyme treatment
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Authors
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A.K. DAS1, D.K. MITRA and Y.S. AHLAWAT
Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012
Keywords:
Brinjal little leaf. phytoplasrna, macerating enzymes, DAPI, fluorescence microscopyAbstract
Phloem elements of little leaf infected aubergine plants were separated from other cell types by partial digestion of plant tissue with macerating enzymes and viewed by fluorescence microscopy after staining with the fluorochrome, 4', 6-diamidino-2-phenylindole (DAPI). The isolated phloem elements from infected plants showed typical fluorescence indicating the presence of the causal phytoplasma. No such specific fluorescence was observed in healthy phloem tissues.
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