Some biological and genomic properties of pigeonpea isolate of mungbean yellow mosaic geminivirus
330 / 245
Authors
-
BIKASH MANDAL, ANUPAM VARMA and V.G. MALATHI
Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute,
New Delhi 110012
Keywords:
Pigeon pea, mungbean yellow mosaic geminivirus, molecular cloning, DNA probe detectionAbstract
The causal virus of yellow mosaic of pigeon pea (Cajanus cajan) was found to be an extended host range phenotype of mungbean yellow mosaic geminivirus (MYMV). The pigeonpea isolate (MYMV-Pp) could be transmitted by whitefly (Bemisia tabaci) to mungbean (Vigna radiata), blackgram (Vigna mungo), Frenchbean (Phaseolus vulgaris), soybean (Glycine max), Iimabean (Phaseolus lunatus), horsegram (Macrotyloma unijlorum) and cowpea (Vigna unguiculata). Double stranded DNA forms (open circular, linear and supercoiled) from infected plants were purified and cloned in to pUC18 and pBluescript SK+ vectors; four full length clones were obtained. All the four clones obtained were found to be D A-B on the basis of hybridization with MYMV-Bg DNA-B. Restriction analysis of clone pMY-PB3 revealed that it had a pattern similar to that of MYMV-Bg (blackgram isolate) D A-B, suggesting that MYMV-Bg and MYMV-Ppl are closely related. D A-B of MYMV-Ppl, however, differed considerably in its restriction maps from that of MYMV-T, a mechanically transmissible isolate characterised from Thailand. D A-B probes detected MYMV-Ppl in the plants, and in adults and pupae of viruliferous whiteflies.
Downloads
Issue
Section
License
For Authors
As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.
For Readers
While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.
All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.