Genetic diversity analyses of Mycosphaerella eumusae causing Septoria leaf spot disease of banana in India


409 / 436

Authors

  • P. GANGA DEVI and R. THANGAVELU*

Keywords:

Banana, genetic diversity, leaf spot disease, Mycosphaerella eumusae

Abstract

The leaf spot diseases caused by Mycosphaerella spp. are considered as one of the major constraints of banana production in entire banana growing regions of India. To identify the major leaf spot pathogen, a total of 99 isolates of leaf spot pathogen were isolated from different banana growing states. All these isolates were identified as M. eumusae based on cultural and morphological characters of the fungus. For confirmation, the rDNA-ITS region of all the 99 isolates of M. eumusae was sequenced and the Blast analysis showed 99-100% similarity only with M. eumusae, which causes Septoria leaf disease in banana. Further, the genetic diversity analyses carried out using the sequence data of rDNA-ITS region and by RAPD indicated the presence of 8-12 haplotypes among the M. eumusae isolates and also the existence of 98-100% homology between the three species of Mycosphaerella (M. musicola, M. fijiensis and M. eumusae) which are the major leaf spot disease causing pathogens in different banana growing regions of the world. This suggests that all these three species of Mycosphaerella have been derived from a common ancestor. The phylogenetic analysis also indicated that both these molecular methods have also clearly distinguished all the three species of Mycosphaerella by grouping them separately but in the major sub cluster of either M. musicola or M. fijiensis. This paper reports for the first time, the genetic diversity analysis carried out among the wide population of M. eumusae isolates that are causing severe yield and quality loss to banana in India.

Downloads

Issue

Vol. 67 No. 4 (2014): Indian Phytopathology

Section

Research Articles

License

For Authors

As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.

For Readers

While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.

How to Cite

R. THANGAVELU*, P. G. D. and. (2014). Genetic diversity analyses of Mycosphaerella eumusae causing Septoria leaf spot disease of banana in India. Indian Phytopathology, 67(4), 388-398. https://epubs.icar.org.in/index.php/IPPJ/article/view/45502