Comparative Efficacy of Three Stains for the Diagnosis of Canine Dermatophytosis
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Authors
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V. Arun Prakash
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala
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R. L. Rathish
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala
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P. M. Deepa
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala
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K. C. Bipin
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala
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L. John
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala
Keywords:
Calcofluor white, Chicago sky blue, Dermatophytosis, MicrosporumAbstract
Dermatophytosis was the predominant mycological infection in small animals characterised by a superficial skin infection restricted to keratinized epithelium caused by fungal species belonging to the genera Microsporum, Trichophyton and Epidermophyton characterised by generalised alopecia and scaling. A total of 114 dogs with skin lesions presented at Teaching Veterinary Clinical Complex, Pookode, Wayanad from July 2021 to January 2022 were tested for dermatophytosis by culturing in dermatophyte test medium (DTM) and etiologies were identified by Lactophenol Cotton Blue, Chicago sky blue 6B and Calcofluor white staining. Efficacy of three stains in different external and internal features of fungal structures were compared by observing the mounts after allowing the stains to react for five minutes, 15 minutes and 30 minutes. Chicago sky blue staining was more efficient for the visualization of morphological and internal features of the fungi, when stain is allowed to react for 30 minutes.
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References
Afshar, P., Larijani, L.V. and Rouhanizadeh, H. (2018) A comparison of conventional rapid methods in diagnosis of superficial and cutaneous mycoses based on KOH, Chicago sky blue 6B and calcofluor white stains. Iran. J. Microbiol. 10: 433.
Attal, R. O., Chaudhary, R.A. and Deotale, V.S. (2015) A clinico-mycological study of onychomycosis in a rural hospital in central India. Int. J. Res. Med. Sci. 3:1131–1137.
Chandrahas Sannat, M., Kalim, O., Hirpurkar, S.D., Rawat, N., Jolhe, D., Gupta, A.K.,Gumasta, P. and Kamble, M.V. (2019) Isolation of Microsporum canis from dog and its therapeutic management. J. Entomol. Zool. 7: 516-518.
Copetti, M.V., Santurio, J.M., Cavalheiro, A.S., Boeck, A.A., Argenta, J.S., Aguiar, L.C. and Alves, S.H. (2006) Dermatophytes isolated from dogs and cats suspected of dermatophytosis in Southern Brazil. Acta. Sci. Vet. 34:119-124.
Debnath, C., Mitra, T., Kumar, A. and Samanta, I. (2016) Detection Of dermatophytes in healthy companion dogs and cats in eastern India. Iran. J. Vet. Res. 17:20.
Didehdar, M., Shokohi, T., Khansarinejad, B., Sefidgar, S.A.A., Abastabar, M., Haghani, I., Amirrajab, N. and Mondanizadeh, M. (2016) Characterization of clinically important dermatophytes in North of Iran using PCR-RFLP on ITS region. J. Mycol. Med. 26: 345-350.
Eiashmawy, W.R. and Ali, M.E. (2016) Identification of different dermatophytes isolated from cattle, cats and horses suffered from skin lesions. Alex. J. Vet. Sci.49: 126-132.
Khan, S.A., Xess, I. and Rai, A. (2015) Calcofluor white stain; A rapid detection system for nosocomial fungal infection. Indian. J. Appl. Res. 5: 692-694.
Mourad, B., Ismail, M., Hawwam, S., Msseha, M. and Hassan, R. (2019) Evaluation of the efficacy of fluorescent staining and chicago sky blue staining as methods for diagnosis of dermatophytosis in hair and nails. Clin. Cosm. Invest. Dermatol. 12:751.
Parmar, B.C., Nayak, J.B., Brahmbhatt, M.N., Chaudhary, J.H., Patel, S.A. and Gida, H.K. (2018) Prevalence of dermatophytosis in animal and human population with special reference to its zoonotic significance. Int. J. Pure App. Biosci. 6: 687-691.
Prakash, R., Prashanth, H.V., Ragunatha, S., Kapoor, M., Anitha, T. K. and Krishnamurthy, V. (2016) Comparative study of efficacy, rapidity of detection,and cost effectiveness of potassium hydroxide, calcofluor white, and Chicago skyblue stains in the diagnosis of dermatophytoses. Int. J. Dermatol. 55: 172-175.
Quinn, P. J., Markey, B. K., Leonard, F. C., Patrick, E. S. F., Fanning, S. and Hartigan,P. J. (2011) Veterinary microbiology and microbial diseases. (2nd Ed). Wiley Blackwell, 928p.
Shwetha, J.V., Harsha, T.R., Shariq, A.K.(2017) Comparison of Chicago sky blue (novel stain) with Calcofluor white and potassium hydroxide mount for rapid diagnosis of dermatomycosis and onychomycosis in a tertiary care centre. Int. J. Curr. Res. 9:46864–46868.
Singh, T.N., Zamzachin, G. and Singh, N.B. (2016) Recognition of dermatophytes by dermatophyte test medium. Int. J. Curr. Microbiol. Appl. Sci. 5: 1125-1129.