Persistent Carrier Status Of Ehrlichiosis And Babesiosis In A German Shepherd Dog After Clinical Recovery
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Authors
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P.I.Ganesan
Department of Veterinary Medicine, Apollo College of Veterinary Medicine, Jaipur
Keywords:
Ehrlichiosis, Babesiosis, German ShepherdAbstract
Vector- borne diseases of canines are common all over the globe and their prevalence is high in India due to the hot and humid climatic conditions. Among prevailing vector- borne diseases canine ehrlichiosis, babesiosis and hepatozoonosis are commonly reported, followed by anaplasmosis and trypanasomiasis. A seven months old German shepherd male dog brought to the VeterinaryClinical Complex of Apollo College of Veterinary Medicine, Jaipur. The clinical signs exhibited by the dog were anorexia for 45days, congested mucus membrane, transient fever, and enlarged popliteal lymphnode. The dog was treated in various clinics using cefotaxime and other supportive drugs without success.Dog infested withRhipicephalus sanguineous was confirmed. Blood smear ex amination revealed a mixed infection of E.canis and B.bigemina on zero day of admission. Hematological tests revealed alterations in the haemogram. Accordingly, the dog was treated with single dose of diminazeneaceturate on first day, followed with Doxycycline for a period of 14 days and the dog recovered from the clinical signs. The body weight of the dog increased 1.5 kg during this treatment period and the dog was in normal habits. Blood smear examination of the dog on 26th and 32nd day post treatment confirmed the presence of both B.gibsoni and E.canis showing the resilience status for B.gibsoni and E.canis parasites after the treatment regimen, denoting persistent carrier status of both B.gibsoni and E.canisin the infected dog.
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References
Allsopp, M.T.E.P. and Allsopp, B.A. (2006). Molecular sequence evidence for the re-classification of some Babesia species. Annals of New York Academic Science, 1081: 509–17.
Brown GK, Canfield PJ, Dunstan RH, Roberts TK, Martin AR, and Brown CS, (2006). Detection of and their impact on platelet numbers in free-roaming dogs associated with remote Aboriginal communities in Australia. Anaplasma platys Babesiacanisvogeli Aust Vet J 84(9): 321-325.
Collect, M.G., (2000). Survey of canine babesiosis in South Africa, Journal of the South African Veterinary Association 71 : 180-186.
Dantas-Torres F. (2008) The brown dog tick, (Latreille, 1806) (Acari: Ixodidae): from taxonomy to control. Rhipicephalussanguineus VetParasitol; 152(3-4) : 173-185.
Das, M. and Konar, S. (2013). Clinical and hematological study of canine ehrlichiosis with other hemoprotozoan parasites in Kolkata, West Bengal, India. Asian Pacific Journal of Tropical Biomedicine, 3(11) : 913–915
Dhankar, S., Sharma, R.D. and Jindal, N. (2011). Someepidemiological observations on canine ehrlichiosis in Haryana and Delhi State. Haryana Veterinarian, 50 : 9-14
Edward B Breitschwerdt (2011). World Small Animal Veterinary Association World Congress Proceedings. College of Veterinary Medicine North Corolina State University.USA.
Gal A, Loeb E, Yisaschar-Mekuzas Y, and Baneth G. (2008) Detection of by PCR in different tissues obtained during necropsy from dogs surveyed for naturally occurring canine monocyticehrlichiosis. (2008) Ehrlichiacanis Vet J; 175(2) : 212
Irwin PJ.(2009). Canine babesiosis: from molecular taxonomy to control. Parasit Vectors; 2 (Suppl 1): S4.
Iqbal, Z. and Rikihisa, Y. (1994). Re-isolation of Ehrlichiacanis from blood and tissues of dogs after doxycycline treatment. Journal of Clinical Microbiology, 32(7) : 1644–1649.
Jain NC (1993). Essentials of veterinary hematology. Philadelphia, Lea & Fabiger
Jayabal L., Nagarajan N K and Ramnesh K. (2019). Concurrent infection of Babesiacanis and Ehrlichiacanis in a Labrador Retriver dog.11 (2) : 174- 178.
Jadhav, R.K., Kumari, R., JavedJameel, A. and Pankaj Kumar (2011). Emergence of arthropod transmitted infections in kennel dogs, Veterinary World, 4(11): 522-528.
Jain N C. (1993) Essentials of veterinary hematology Philadelphia: Lea &Febiger
Jefferies R, Ryan UM, Jardine J, Robertson ID, and Irwin PJ. (2007) Babesiagibsoni: detection during experimental infections and after combined atovaquone and azithromycin therapy. Exp Parasitol.; 117:115–23.
Johnson, E.M., Ewing, S.A., Barker, R.W., Fox, J.C., Crow,D.W. and Kocan, K.M. (1998). Experimental transmission of Ehrlichiacanis (Rickettsials: Ehrlichieae) byDermacentorvariabilis (Acari:Ixodidae). Veterinary Parasitology, 31: 277–288.
Kelly P.J. (2000). Canine ehrlichiosis: An Update. Journal of the South African Veterinary Association, 71: 77-86.
Kettner , F., Reyers, F. and Miller, D. (2003). Thrombocytopenia in canine babesiosis and its clinicalk usefulness. Journal of the Southern African Veterinary Association, 74: 63-68.
Lin MY, and Huang HP.(2010) Use of a doxycycline-enrofloxacin-metronidazole combination with/without diminazene di-aceturate to treat naturally occurring canine babesiosis caused by Babesiagibsoni. Acta Vet Scand.: 52:27.
Lin EC, Chueh LL, Lin CN, Hsieh LE, and Su BL (2012) The therapeutic efficacy of two anti babesial strategies against Babesiagibsoni. Vet Parasitol.; 186 : 159–64.
Lakshmanan, B., John, L., Dhinakarraj, G. and Gomathinayagam, S. (2011). Early diagnosis of canine ehrlichiosis by hot-start P C R. Journal of Applied Animal Research, 31: 11–12.
McClure J C, Crothers M C,Schaefer J J, Stanley P D, Needham G R, and Ewing S A. (2010). Efficacy of doxycycline treatment regiemen initiated during three different phases of experimental ehrlichiosis. Antimicrob Agents chemother. 54 (12) : 5012-20.
Mylonakis, M.E., Siarkou, V.I. and Koutinas, A.F. (2011). Myelosuppressivecaninemonocyticehrlichiosis (Ehrlichiacanis): An update on the pathogenesis, diagnosis and management. Israel Journal of Veterinary Medicine, 65: 129-135.
Miller DM, Swan GE, Lobetti RG, and Jacobson LS. (2005). The pharmacokinetics ofdiminazeneaceturate after intramuscular administration in healthy dogs. J S Afr Vet Assoc. 76 : 146–50
Neer, T.M. (1998) Canine monocytic and granulocytic ehrlichiosis In: G.E.Greene, Ed., Infectious diseases of the dog and cat. 2ndedi. W.B. Sanders Co., Philadelphia, pp:139-147.
Nyindo, M., Huxsoll, D.L. and Ristic, M. (1980). Cell-mediated and humoral immune responses of German Shepherd dogs and Beagles to experimental infection with Ehrlichiacanis . American Journal of Veterinary Research, 41: 250–254.
Passos LM, Geiger SM, Ribeiro MF, Pfister K, and Zahler-Rinder M. (2005). First molecular detection of in dogs from Brazil. Babesiavogeli VetParasitol 127(1): 81-85
Ramos R, Ramos C, Araújo F, Oliveira R, Souza I, and Pimentel D (2010) Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). Parasitol Res; 107(5): 1115-1120.
Rautenbach, G.H., Boomker., J and De Villiers, I.L (1991). A descriptive study of the canine population in a rural town in Southern Africa, Journal of the South African Veterinary Association 62 : 158-162.
Santos F, Coppede JS, Pereira AL, Oliveira LP, Roberto PG, and Benedetti RB, (2009). Molecular evaluation of the incidence of Ehrlichiacanis, Anaplasmaplatys and spp. in dogs from RibeirãoPreto, Brazil. Babesia Vet J ; 179(1): 145-148.
Sainz, A., Roura, X., Miro, G., Estrada-Pena, A. and Kohn, B.(2015). Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Parasites Vectors, 8: 1-20.
Saravanan, S., Palanivel, K.M., Senthilvel, K. and Sathyabama,T.B. (2014). Babesiacanis infection in a Labrador pup –A clinical case report. Indian Journal of Veterinary Medicine, 34: 152-153.
Sakuma M, Setoguchi A, and Endo Y. (2009) possible emergence of drug-resistant variants of Babesiagibsoni in clinical cases treated with atovaquone and azithromycin. J Vet Intern Med. 23: 493–8.
Scheepers, E., Leisewitz, A.L., Thompson, P.N. and Christopher, M.M. (2011). Serial haematology results in transfused and non-transfused dogs naturally infected with Babesia Rossi. Journal of the South African Veterinary Association, 82: 136–143.
Schetters, T.P.M., Kleuskens, J.A.G.M., van de Crommert, J de Leeuw, P.W.J., Finizio, A.L. and Gorenflot, A.(2009). Systemic inflammatory responses in dogs .Experimentally infected with Babesiacanis; ahaematological study. Veterinary Parasitology, 162(1–2): 7–15.
Schouls, L.M., Van De Pol, I., Rijpkema, S.G. and Schot, C.S. (1999). Detection and identification of Ehrlichia, Borreliaburgdorferisensulato, and Bartonella species in Dutch Ixodesricinus ticks. Journal of Clinical Microbiology, 37: 2215–2222.
Shaw, S.E., Day, M.J., Birtles, R.J. and Breitschwerdt, E.B.(2001). ‘Tick-borne infectious diseases of dogs. Trends in Parasitology,17: 74-80.
Shipov, A., Klement, E., Reuveni-Tager, L., Waner, T. and Harrus, S. (2008). Prognostic indicators for canine monocyticehrlichiosis. Veterinary Parasitology, 153:131–138.
Singla, L.D.,Singh, H., Kaur, P., Singh, N.D., Singh, N.K. and Juyal, P.D. (2011). Sero-detection of Ehrlichiacanis infection in dogs from Ludhiana district of Punjab. Indian Journal of Parasitic Diseases, 35(2): 195–198.
Spolidorio MG, Labruna MB, Zago AM, Donatele DM, Caliari KM, and Yoshinari NH.(2009) Infecting dogs in the State of Espírito Santo, southeastern Brazil. (2009) Hepatozooncanis VetParasitol; 163(4): 357-361.
Spolidorio MG, Torres MM, Campos WN, Melo AL, Igarashi M, and Amude AM, (2011) Molecular detection of and in domestic dogs from Cuiabá, Brazil. Hepatozooncanis Babesiacanisvogeli Rev Bras Parasitol Vet; 20(3): 253-255.
Solano-Gallego, L. and Baneth, G. (2011). Babesiosis in dogs and cats - expanding parasitological and clinical spectra. Veterinary Parasitology, 181: 48–60.
Trapp SM, Dagnone AS, Vidotto O, Freire RL, Amude AM, and de MoraisHS.(2006). Sero-epidemiology of canine babesiosis and ehrlichiosis in a hospital population. Vet Parasitol 140 (3-4): 223-230.
Tereza Emmanuelle de FarisRotondano., HertaKaryaanneAraujoAlmeda., Felipe da Silva Krawczak.,Vanessa Lira Santana., IvanaFernandes Vidal., Marcela Bahia Labruna., Sergio Santos de Azevedo., Alzira Maria Paiva de Almeida., and Marcia Almeida de Melo (2015). Survey of Ehrlichiacanis, Babesia spp. and Hepatozoon spp. in dogs from a semiarid region of Brazil.Rev.Bras.Parasitol.Vet. 24 (1)
Tereza Emmanuelle de FarisRotondano (2015).
Toepp AJ., Ugwu CA., Petersen CA., and Sykes J E (2017). Detection and identification of blood borne infections in dogs in Nigeria using light microscopy and the polymerase reaction.Veterinary Parasitology, Regional Studies Reports, 19 (11): 55-60.
Wulansari R, Wijaya A, Ano H, Horii Y, and Nasu T, (2003). Clindamycin in the treatment of Babesiagibsoni infections in dogs. J Am AnimHosp Assoc.; 39:558–62.
YolandiRautenbach., Johan Schoeman., and Amelia Goddard (2018) Prevalence of canine babesia and ehrlichia co-infection and the predictive valve of hematology. Onderstepoort Journal of Veterinary Research 85 (1) : 1-5.
