Assessment of Acoustic Pulse Technology (APT-X) in the treatment of Mastitis in Indian Dairy Cows – A Multicentered Trial compared to standard Antibiotic Treatment

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  • Gabriel Leitner Armenta Ltd
  • D. N. Hegde Consultant KMF, Ex- Director AH, KMF
  • Vikas Gupta Advisor- Indian Veterinary Association
  • Ragothaman S Director – Regenta Biodiagnostics Private Limited
  • Narendrakumar R Director – Regenta Biodiagnostics Private Limited


Acoustic Pulse Technology, Mastitis, Indian Dairy Cows, Antibiotic Treatment


India is the world’s largest milk-producing country, with a 20% share in global production (OECD 2019) with over 193 million cattle .In India cattle are sacred, thus many states do not permit the slaughter of dairy cattle for meat production (Sharma et al., 2019). Mastitis, the inflammation of the mammary gland, is the single most important factor that imposes economic burdens on dairy farms worldwide (Halasa et al., 2007). Estimations show that 30% of dairy cattle are affected in both forms of mastitis infection, i.e., clinical (CM) and subclinical (SCM), and it is the most common reason for using antibiotics in dairy cows as preventive and treatment measures (Halasa et al., 2007; Pol and Ruegg, 2007; Saini et al. 2012). Antibiotics have been used to treat bovine mastitis for more than half a century, however, the effectiveness, financial aspects and long-term effects are debatable. Growing awareness of spread of antimicrobial-resistant (AMR) bacteria are influencing the decision-making progress of reducing antibiotics used in livestock production (Economou and Gousia, 2015; Magouras et al., 2017). Therefore, judicious use of mastitis antibiotics treatment should be evidence-based (Pyörälä, 2009).Continued use of antibiotics in the treatment and prevention of mastitis has a direct effect on fostering AMR, which has hazardous consequences on human health (Sawant et al., 2007). Moreover, antibiotics might weaken the cows’ immune system, making it more susceptible to repeated infections (Bradley et al., 2007; Enli et al., 2019).


Bradley, A.J., Leach, K.A., Breen, J.E., Green, L.E., and Green, M.J., (2007) Survey on the incidence and aetiology of mastitis on dairy farms in England and Wales. Vet. Rec. 160 : 253–257.

d’Agostino, M.C., Craig, K., Tibalt, E., and Respizzi, S., (2015) Shock wave as biological therapeutic tool: from mechanical stimulation to recovery and healing, through mechanotransduction. Int. J. Surg. 24:147-153.

Davis, T.A., Stojadinovic, A., Anam, K. Amare, M., Naik, S., Peoples, G.E., Tadaki, D., and Elster, E.A., (2009) Extracorporeal shock wave therapy suppresses the early pro inflammatory immune response to a severe cutaneous burn injury. Int. Wound J. 6: 11-21.

Economou, V., and Gousia, P., (2015) Agriculture and food animals as a source of antimicrobial-resistant bacteria. Infect. Drug Resist. 8 : 49-61.

Enli, L., Lai, K.S., and Mansor, R., (2019) Antimicrobial usage and resistance in dairy cattle production. Vet. Med. Pharm. Intech Open. DOI: 10.5772/intechopen.81365

Halasa, T., Huijps, K., Østerås, O., and Hogeveen, H., (2007) Economic effects of bovine mastitis and mastitis management: A review. Vet. Quart. 29 : 18-31.

Leitner, G., Zilberman, D., Papirov, E., and Shefy, S., (2018) Assessment of Acoustic Pulse Therapy (APT), a non-antibiotic treatment for dairy cows with clinical and subclinical mastitis. PLoS One, 13, e0199195.

Leitner, G. Papirov, E. Gilad, D. Haran, D. Arkin, O. 3, Zuckerman, A. and Lavon, Y. (2021). New Treatment Option for Clinical and Subclinical Mastitis in Dairy Cows Using Acoustic Pulse Technology (APT). Dairy 2 : 256–269.

Magouras, I., Carmo, L.P., Stärk, K.D., and Schüpbach-Regula, G., (2017) Antimicrobial usage and-resistance in livestock: where should we focus? Front. Vet. Sci. 4 : 148.

Mariotto, S., de Prati, A.C., Cavalieri, E., Amelio, E., Marlinghaus, E., and Suzuki, H., (2009). Extracorporeal shock wave therapy in inflammatory diseases: molecular mechanism that triggers anti-inflammatory action. Curr. Med. Chem. 16 : 2366-2372.

Merin,U. Leitner, G. Jacoby, S. and Gilad, D. (2021). Management of high cows-share-contribution of SCC to the bulk milk tank by acoustic pulse technology (APT). PLoS ONE.

OECD and FAO 2018 OECD-FAO Agricultural Outlook 2018–2027. Chapter 7. Dairy and Dairy Products. [(accessed on 26 September 2019)]; Available online:

Pol, M., and Ruegg, P.L., (2007). Treatment practices and quantification of antimicrobial drug usage in conventional and organic dairy farms in Wisconsin. J. Dairy Sci. 90 : 249-261.

Pyörälä, S., (2009). Treatment of mastitis during lactation. Ir. Vet. J. 62 : S40–S44.

Rohringer, S., Holnthoner, W., Hackl, M., Weihs, A.M., Rünzler, D., Skalicky, S., Karbiener, M., Scheideler, M., Pröll, J., Gabriel, C., Schweighofer, B., Gröger, M., Spittler, A., Grillari, J., and Redl, H., (2014). Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells. PloS ONE, 9, e114806.

Saini, V., McClure, J.T., Leger, D., Dufour, S., Sheldon, A.G., Scholl, D.T., and Barkema, H.W., (2012). Antimicrobial use on Canadian dairy farms. J. Dairy Sci. 95 : 1209-1221.

Sawant, A.A., Hegde, N.V., Straley, B.A., Donaldson, S.C., Love, B.C., Knabel, S.J., and Jayarao, B.M., (2007). Antimicrobial-resistant enteric bacteria from dairy cattle. Appl. Environ. Microbiol. 73 : 156-163. DOI:10.1128/AEM.01551-06

Sharma A., Kennedy U., Schuetze C., and Phillips C.J.C. (2019) The welfare of cows in Indian shelters. Animals. 9 : 172. doi: 10.3390/ani9040172.

Waugh, C., Morrissey, D., Jones, E., Riley, G., Langberg, H., and Screen, H., (2015). In vivo biological response to extracorporeal shockwave therapy in human tendinopathy: Response of tendinopathy to shockwave therapy. Eur. Cells Mater. 29 : 268-280.

Yahata, K., Kanno, H., Ozawa, H., Yamaya, S., Tateda, S., Ito, K., Shimokawa, H., and Itoi, E., (2016). Low-energy extracorporeal shock wave therapy for promotion of vascular endothelial growth factor expression and angiogenesis and improvement of locomotor and sensory functions after spinal cord injury. J. Neurosurg. –Spine, 25 :, 745-755.








How to Cite

Assessment of Acoustic Pulse Technology (APT-X) in the treatment of Mastitis in Indian Dairy Cows – A Multicentered Trial compared to standard Antibiotic Treatment. (2023). The Indian Veterinary Journal, 100(11), 35-39.