Greenwater Technology for Controlling Vibriosis and Achieving Higher Shrimp Production - A Case Study
Disease due to luminous Vibrio has been a major problem in the shrimp industry. Brackishwater shrimp Penaeus monodon is susceptible to fatal effects of luminous vibriosis adversely affecting the shrimp production and creating economic losses in India. Different technologies have been introduced to control the disease. One of the techniques reported to work against luminous bacteria is greenwater culture system (or finfish–shrimp integrated culture system). The greenwater culture system has been proven the most functional and innovative technique, wherein euryhaline finfish are propagated as bioremediators in an isolated net pens/cages inside the shrimp growing ponds or shrimp are cultured in fish produced greenwater. This paper reports a case study on the successful demonstration of greenwater technology based on the integration of milkfish, Chanos chanos and grey mullet, Mugil cephalus in pens for environmental and health management of brackishwater aquaculture with the result of higher production of shrimp, Penaeus monodon. The greenwater technology has been found to be effective as biomanipulators in controlling the luminous bacteria disease and can be a good alternative to expensive microbial products/probiotics in the aqua-farming ponds throughout the culture period.
Anand, T.P., Bhat, A.W., Shouche, Y.S., Roy, U., Siddharth, J. and Sarma, S.P. (2006). Antimicrobial activity of marine bacteria associated with sponges. Microbial Research 161: 252-262. https://doi.org/10.1016/j.micres.2005.09.002
Andlid, T., Juarez, R.V., and Gustafsson, L. (1995). Yeast colonizing the intestine of rainbow trout(Salmo gairdneri) and turbot (Scophthalmus maximus). Microbial Ecology 30: 321–334. https://doi.org/10.1007/BF00171938
APHA. (1989). Standard Methods for the Examination of Water and Wastewater. American Public Health Association (APHA), Washington, D.C., USA.
Baliao, D.D. (2000). Environment-friendly Schemes in Intensive Shrimp Farming. Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, Philippines. 24 p. http://hdl.handle.net/10862/1544
Baliao, D.D., de los Santos, M.A. and Franco, N.M. (1999). The modular method: Milkfish pond culture. Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, Philippines. http://hdl.handle.net/10862/1545
Blunt, J.W., Copp, B.R., Hu, W.P., Munro, M.H., Northcote, P.T. and Prinsep, M.R. (2007). Marine natural products. Natural Products Reports 24: 31-86. https://doi.org/10.1039/B603047P
Boyd, C.E. (2009). Phytoplankton in aquaculture ponds. Global Aquaculture Advocate Jan-Feb: 65-66.
Crab, R., Lambert, A., Defoirdt, T., Bossier, P., and Verstraete, W. (2010). The application of bioflocs technology to protect brine shrimp (Artemia franciscana) from pathogenic Vibrio harveyi. Journal of Applied Microbiology 109(5): 1643-1649. https://doi.org/10.1111/j.1365-2672.2010.04791.x
Cremen, M.C.M., Martinez-Goss, M.R., Corre, V.L. and Azanza, R.V. (2007). Phytoplankton bloom in commercial shrimp ponds using green-water technology. Journal of Applied Phycology 196: 615-624. https://doi.org/10.1007/s10811-007-9210-7
Dash, P., Ayunje, S., Tandel, R.S., Sandeep, K.P. and Panigrahi, A. (2017). Biocontrol of luminous vibriosis in shrimp aquaculture: A review of current approaches and future perspectives. Reviews in Fisheries Science and Aquaculture 25(3): 245-255. https://doi.org/10.1080/23308249.2016.1277973
Decamp, O. (2004). Probiotics in aquaculture: A commentary based on some recent observations. Aqua Feeds: Formation & Beyond 1(4): 12-13.
Garriques, D. and Arevalo, G. (1995). An evaluation of the production and use of a live bacterial isolate to manipulate the microbial flora in the commercial production of Penaeus vannamei post larvae in Ecuador. In: Swimming Through Troubled Water, C.L. Browdy and J.S. Hopkins (eds.), Proceedings of the special session on shrimp farming, Aquaculture 1995, World Aquaculture Society, Baton Rouge, LA, USA. pp 53-59.
Karunasagar, I., Pai, R., Malathi, G.R. and Karunasagar, I. (1994). Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio haeveyi infection. Aquaculture 128: 203-209.
Kathiravan, V., and Krishnani, K.K. (2012). Diversity of diazotrophic bacteria in greenwater system of coastal aquaculture. Journal of Environmental and Applied Bioresearch 1(1): 5-11.
Kathiravan, V., Krishnani, K.K., Azad, I.S., Kailasam, M. and Ponniah, A.G. (2012). Diversity of nitrite oxidizers in greenwater system of coastal aquaculture by metagenomic approach targeting nxrb genes. Biotechnology: An Indian Journal 6(11): 359-366.
Kathiravan, V. and Krishnani, K.K. (2013). Heterotrophic nitrifying and oxygen tolerant denitrifying bacteria from greenwater system of coastal aquaculture. Applied Biochemistry and Biotechnology 169(6): 1978-1992. https://doi.org/10.1007/s12010-013-0109-2
Kathiravan, V. and Krishnani, K.K. (2014a). Pseudomonas aeruginosa and Achromobacter sp., nitrifying aerobic denitrifiers have a plasmid encoding for denitrifying functional genes. World Journal of Microbiology and Biotechnology 30(4): 1187-1198.
Kathiravan,V. and Krishnani, K.K. (2014b). Diversity of denitrifying bacteria in greenwater system of coastal aquaculture. International Aquatic Research 6(3): 135-145. https://doi.org/10.1007/s40071-014-0074-6
Krishnani, K.K., Kathiravan, V., Kailasam, M., Nagavel, A. and Ponniah, A.G. (2019). Isolation and characterization of antagonistic bacteria from milkfish Chanos chanos against Vibrio harveyi. Indian Journal of Fisheries 66(1): 124-130.
Krishnani, K.K., Kailasam, M., Kathiravan, V. and Nagavel, A. (2012). Aquatic bioaugmentation in coastal aquaculture. Development and demonstration of greenwater technology. Fishing Chimes 31(10): 76-79.
Krishnani, K.K. (2021). Finfish based bioaugmentation technology for enhancing shrimp grower’s income and livelihood. Agricultural Research 10(3): 361-368. https://doi.org/10.1007/s40003-020-00530-y
Krishnani, K.K., Kathiravan, V., Natarajan, M., Kailasam, M. and Pillai, S.M. (2010). Diversity of sulfur oxidising bacteria in greenwater system of coastal aquaculture. Applied Biochemistry and Biotechnology 162: 1225-1237. https://doi.org/10.1007/s12010-009-8886-3
Lachlan, H., Leigh, O. and Sandra, S.A. (1996). Selective and differential medium for Vibrio arveyi. Applied and Environmental Microbiology 62(9): 3548-3550.
Lightner, D.V. (1996). A Handbook of Shrimp Pathology and Diagnostic Procedures for Disease of Cultured Penaeid Shrimp. World Aquaculture Society, Baton Rouge, LA, USA. 193 p.
Liu, C.H. and Chen, J.C. (2004). Effect of ammonia on the immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus. Fish and Shellfish Immunology 16(3): 321-334. https://doi.org/10.1016/S1050-4648(03)00113-X
Martínez, P.C., Ibáñez, A.L., Monroy Hermosillo, O.A. and Ramírez Saad, H.C. (2012). Use of probiotics in aquaculture. International Scholarly Research Notices 2012: 916845. https://doi.org/10.5402/2012/916845
Moriarty, D.J.W. (1999). Disease control in shrimp aquaculture with probiotic bacteria, Proceedings 8th International Symposium on Microbial ecology, C.R. Bell, M. Brylinsky and P. Johnson-Green (eds), Atlantic Canada Society for Microbial Ecology, August 9-14, 1999, Halifax, Canada.
Strickland, J.D.H. and Parsons, T.R.A. (1972). A Practical Handbook of Seawater Analysis. Bulletin 167, Canadian Government Publishing Centre, Ottawa, Canada.
Tendencia, E.A., Bosma, R.H., Verdegem, M.C.J.,and Verreth J.A.J. (2015). The potential effect of greenwater technology on water quality in the pond culture of Penaeus monodon Fabricius. Aquaculture Research 46: 1-13. https://doi.org/10.1111/are.12152
Tendencia, E.A., Bosma, R.H. and Sorio, L.R. (2012). Effect of three innovative culture systems on water quality and white spot syndrome virus (WSSV) viral load in WSSV fed Penaeus monodon cultured in indoor tanks. Aquaculture 350-353: 169-174. https://doi.org/10.1016/j.aquaculture.2012.04.001
Tendencia, E.A. and dela Pena, M.R. (2010). Effect of different sizes of saline red tilapia hybrid Oreochromis niloticus x O. mossambicus on the growth of luminous bacteria Vibrio harveyi. Philippine Agricultural Scientist 93: 363-467.
Tendencia, E.A., dela Pena, M.R., Fermin, A.C., Lio-Po, G., Choresca, C.H. and Inui, Y. (2004). Antibacterial activity of tilapia Tilapia hornorum against Vibrio harveyi. Aquaculture 232: 145–154. https://doi.org/10.1016/S0044-8486(03)00531-3
Velmurugan, S. and Rajagopal, S. (2009). Beneficial uses of probiotics in mass scale production of marine ornamental fish. African Journal of Microbiology Research 3(4): 185-190.
Wilkins, M.F., Boddy, L., Morris, C.W. and Jonker, R.R. (1999). Identification of phytoplankton from flow cytometry data using radial basis function neural networks. Applied and Environmental Microbiology 65: 4404-4410.
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