Chemical Seed Priming to Improve Salinity Tolerance in Rice


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Authors

  • LIZ J KAPPEN Department of Agronomy, College of Agriculture, Kerala Agricultural University, Vellanikkara, Thrissur, Kerala - 680 654, India
  • DEEPA THOMAS Rice Research Station, Kerala Agricultural University, Vyttila, Kochi, Kerala - 682 019, India
  • P PRAMEELA Department of Agronomy, College of Agriculture, Kerala Agricultural University, Vellanikkara, Thrissur, Kerala - 680 654, India

https://doi.org/10.54894/JISCAR.40.1.2022.116327

Keywords:

Calcium chloride, Chemical priming, Rice, Salinity tolerance

Abstract

A study was conducted at Rice Research Station, Vyttila to assess the effect of seed priming in improving the salinity tolerance of rice varieties. The study consisted of two parts. In the first part, screening of rice varieties for salinity tolerance was done and in the second experiment, the effect of priming on salinity tolerance was assessed.  The first experiment was a laboratory study to screen three rice varieties, namely, Jyothi, Uma and Vyttila-10 at five salinity levels i.e., non-saline, 3 dS m-1, 6 dS m-1, 9 dS m-1 and 12 dS m-1.  Results showed that  Jyothi was susceptible to 6 dS m-1 salinity at 16 days after sowing (DAS). Uma was moderately tolerant and became susceptible to 6 dS m-1 at 21 DAS. Vyttila-10 was highly tolerant to 6 dS m-1 salinity but it was susceptible to the salinity level of 9 dS m-1 at 21 DAS.  In the second experiment, seeds of these varieties were primed with three chemicals namely beta-amino butyric acid @ 1 mmol L-1, calcium chloride @ 2% and sodium nitroprusside @ 100 µM and with water as control.  The seedlings of Jyothi and Uma were raised at salinity levels of 6 dS m-1 and 9 dS m-1, while seedlings of  Vyttila-10 were raised at salinity levels of 9 dS m-1 and 12 dS m-1. The efficacy of each priming chemical was analyzed based on germination percentage, growth parameters and visual salt injury symptoms. Priming seeds with calcium chloride was found to improve the growth and salinity tolerance of rice seedlings and could be a reliable method to combat the salinity stress of rice in the initial stages of crop growth.

References

Afzal, I., But, A., Rehman H.U., Basra, S.M.A and Afzal, A. (2012). Alleviation of salt stress in fine aromatic rice by seed priming. Australian Journal of Crop Science 6(10): 1401-1407.

Ali, M.N., Yeasmin, L., Gantait, S., Goswami, R. and Chakraborty, S. (2014). Screening of rice landraces for salinity tolerance at seedling stage through morphological and molecular markers. Physiology and Molecular Biology of Plants 4: 411-423.

Amirjani, M.R (2011). Effect of Salinity stress on growth, sugar content, pigments and enzyme activity of rice. International Journal of Botany 7: 73-81.

Arif, M.T.U., Roy, P.R., Sohag, A.A.M., Afrin, S., Rady, M.M. and Hossain, M.A. (2018). Exogenous calcium supplementation improves salinity tolerance in BRRI Dhan 28; a salt-susceptible high-yielding Oryza sativa cultivar. Journal of Crop Science and Biotechnology 21: 383-394.

Azaizeh, H., Gunse, B. and Steudle, E. (1992). Effects of NaCl and CaCl2 on water transport across root cells of maize (Zea mays L.) seedlings. Plant Physiology 99(3): 886-894.

Galahitigama,G.A.H. and Wathugala, D.L.(2016). Effects of pre-sowing seed treatments on seed germination and salinity tolerance of rice (Oryza sativa L.) seedlings. International Journal of Agronomy and Agricultural research 9(6): 112-117.

Gregorio, G.B., Senadhira, D. and Mendoza, R.D.(1997). Screening Rice for Salinity Tolerance. Series No.22. International Rice Research Institute (IRRI), Manila, Philippines, 31p.

Hemalatha, G, Renugadevi, J. and Eevera, T. (2017). Seed priming to alleviate the effect of salinity stress in rice. International Journal of Chemical Studies 5(6): 1140-1143.

Hameed, A., Sheikh, M.A., Basra, S.M.A. and Jamil, A. (2013). Sodium nitroprusside (SNP) seed priming improves seed germination and seedling growth in wheat (Triticum aestivum L.) under osmotic stress induced by polyethylene glycol. International Journal of Biology and Biotechnology 9(4): 379-385.

Jisha, K.C. and Puthur, J.T. (2016).Seed priming with beta-amino butyric acid improves abiotic stress tolerance in rice seedlings. Rice Science 23(5): 242-254.

KAU (2014). Research Report, ICAR -100 crore project- sub project Mechanism of salt tolerance and evolution of rice varieties for the coastal agro ecosystem. Kerala Agricultural University, Vellanikkara, Kerala, India.

Kazemi, K. and H. Eskandari. 2011. Effects of salt stress on germination and early seedling growth of rice (Oryza sativa) cultivars in Iran. African Journal of Biotechnology 10: 17789-17792.

Kumar, K., Kumar, M., Kim, S.R., Kim, H., Ryu, H. and Cho, Y.G. (2013). Insights into genomics of salt stress response in rice. Rice 6(1): 27.

Platten, J.D., Egdane, J.A. and Ismail, A.M. (2013). Salinity tolerance, Na+ exclusion and allele mining of HKT1; 5 in Oryzasativa and O. glaberrima: many sources, many genes, one mechanism? Plant Biology 13: 32.

Savvides, A., Ali, S., Tester, M., and Fotopoulos, V. (2016). Chemical priming of plants against multiple abiotic stresses: mission possible? Trends in Plant Science. 21(4): 330-338.

Sheoran, O.P., Tonk, D.S., Kaushik, L.S., Hasija, R.C., and Pannu, R.S. (1998). Statistical Software Package for Agricultural Research Workers. CCSHAU, Hissar, Haryana, India. 139 p.

Thitisaksakul, M., Tananuwong, K. and Shoemaker, C.F. (2015). Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality. Journal of Agricultural and Food Chemistry 63(8): 2296-2304.

Waziri, A., Kumar, P. and Purty, R.S. (2016). Saltol QTL and their role in salinity tolerance in rice. Austin Journal of Biotechnology and Bioengineering 3(3): 1067.

Yousof, F.I. (2013). Effect of rice seed priming with calcium chloride (CaCl2) on germination and seedling vigor under salinity stress. Journal of Plant Production 4(4): 523-535.

Yoshida, S., Forno, D.A., Cock, J.H. and Gomez, K.A.(1976). Laboratory Manual for Physiological Studies of Rice, Third edition, International Rice Research Institute, Los Banos, Phillippines. pp 61-66.

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Submitted

2021-10-01

Published

2022-07-23

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How to Cite

KAPPEN, L. J., THOMAS, D., & PRAMEELA, P. (2022). Chemical Seed Priming to Improve Salinity Tolerance in Rice. Journal of the Indian Society of Coastal Agricultural Research, 40(1), 13-22. https://doi.org/10.54894/JISCAR.40.1.2022.116327
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