Chlorophyll Content and SPAD Value Relationships Between Varying Nitrogen Application and Cultivar in Rice
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Keywords:Chlorophyll a, Chlorophyll b, Total chlorophyll, Linear model, Rice varieties, SPAD value
The SPAD-502 meter is a portable tool that is extensively used for the rapid, accurate and nondestructive measurement of leaf chlorophyll concentrations. Measurements with the SPAD-502 meter produce relative SPAD meter values that are proportional to the amount of chlorophyll present in the leaf. In order to convert these values into absolute units of chlorophyll concentration, calibration curves must be derived and utilized. Here, we present calibration equations for rice that can be used to convert SPAD values into total chlorophyll per unit leaf area (mg cm-2; y = 0.178x - 3.531) or per unit fresh weight of leaf tissue (3.53 mg g-1). These relationships were derived using leaf analysis of two rice genotypes (cv. ‘Manaswinee’ and cv. ‘Hasanta’) raised with three nutrient management practices (100% Soil Test Based Nitrogen Recommendation (STBNR), 75% STBNR + in situ green manuring (GM) of dhaincha
and 50% STBNR + in situ green manuring of dhaincha) under puddled transplanting condition during Kharif seasons of 2018 and 2019 at Agronomy Main Research Farm, OUAT, Bhubaneswar, Odisha, India. Chlorophyll concentrations were determined at active tillering, heading and physiological maturity stages. Our results revealed that the converted SPAD values differed from photometric measurements of solvent-extracted chlorophyll by regression coefficient (r) values ranging from 0.711 to 0.862 and the relationship was linear using different models. Application of 75% Soil Test Based Nitrogen Recommendation (75 kg N ha-1) with green manuring of dhaincha in rice showed the maximum linear regression coefficient (r=0.862) in rice genotype ‘Hasanta’ at the heading stage.
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