Effect of Organophosphate, Monocrotophos on Behavioural, Haematological, Histological and Biochemical Indices in the Subtropical Catfish Heteropneustes fossilis (Bloch, 1794)

S.T. PATTNAIK; SURYASIKHA SAMAL; C.S.K. MISHRA; PULAK GHOSH; NISHIGANDHA MUDULI; MANISHA BISWAL; PRATIK ACHARYA; RAMJANUL HAQUE; RAJESH KUMAR; JITENDRA KUMAR SUNDARAY

doi:10.54894/JISCAR.40.1.2022.119253

Authors

S.T. PATTNAIK Department of Zoology, Odisha University of Agriculture & Technology Bhubaneswar - 751003, Odisha, India
SURYASIKHA SAMAL Department of Zoology, Odisha University of Agriculture & Technology Bhubaneswar - 751003, Odisha, India
C.S.K. MISHRA Department of Zoology, Odisha University of Agriculture & Technology Bhubaneswar - 751003, Odisha, India
PULAK GHOSH Department of Zoology, Odisha University of Agriculture & Technology Bhubaneswar - 751003, Odisha, India
NISHIGANDHA MUDULI Department of Zoology, Odisha University of Agriculture & Technology Bhubaneswar - 751003, Odisha, India
MANISHA BISWAL Department of Zoology, Odisha University of Agriculture & Technology Bhubaneswar - 751003, Odisha, India
PRATIK ACHARYA Department of Zoology, Odisha University of Agriculture & Technology Bhubaneswar - 751003, Odisha, India
RAMJANUL HAQUE ICAR-Central Institute of Fisheries Education, Versova Mumbai - 400 061, Maharashtra, India
RAJESH KUMAR ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga Bhubaneswar - 751 002, Odisha, India
JITENDRA KUMAR SUNDARAY ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga Bhubaneswar - 751 002, Odisha, India

https://doi.org/10.54894/JISCAR.40.1.2022.119253

Keywords:

Catfish, Haematology, Histology, Monocrotophos, Oxidative stress, Toxicity

Abstract

A study was conducted to evaluate the impact of acute sub-lethal toxicity of monocrotophos on behavioural, biochemical, haematological and histopathological changes in Heteropneustes fossilis (49.53±0.71 g). Probit analysis showed the 96 h LC50 of monocrotophos for H. fossilis was 20 ppm. Followed by the LC₅₀ value, sub-lethal concentrations for acute exposure of monocrotophos were 3 ppm in T₁, 6 ppm in T₂ and 8 ppm in T₃ for 72 h of the experimental period. The behavioural responses observed in treated fish were: erratic movement, imbalance in swimming, surfacing, and hyperactivities. A gradual reduction in total RBC count, haemoglobin, monocyte, and basophil contents were observed with an increased concentration of monocrotophos. On other hand, the total WBC count, neutrophil, basophil and blood ESR showed reversed trend (p<0.05). Total tissue protein content of gill, liver and kidney was altered and decreased significantly (p<0.05) in monocrotophos-treated fish. Tissue lipid peroxidation (LPO) and catalase activities in gill, liver and kidney were also altered after 24 h, 72 h and followed an increasing trend in exposed fish which differed significantly (p<0.05) from the control group. Compared to the control, significant changes were observed in the histopathological architecture of blood cells and gill tissue. The overall result showed that exposure to monocrotophos severely affects fish behaviour and physiology. Therefore the misuse of the chemical may be avoided to reduce the negative impact on aquatic animals.

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