Isolation and Characterization of Beneficial Bacterium from the Rhizosphere of Suaeda nudiflora in Sundarbans, India


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Authors

  • SONALI BANERJEE Ramkrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata - 700 103, West Bengal, India
  • ANANNYA BANERJEE Institute of Agricultural Science, University of Calcutta, Kolkata - 700 019, West Bengal, India
  • ARITRA KUMAR MUKHERJEE Department of Agricultural Chemistry and Soil Science, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur - 741 252, West Bengal, India
  • SUDIPTA TRIPATHI Institute of Agricultural Science, University of Calcutta, Kolkata, West Bengal
  • GAUTAM CHATTERJEE Ramkrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata - 700 103, West Bengal, India

https://doi.org/10.54894/JISCAR.40.2.2022.123565

Keywords:

Nitrogen fixation, Phosphate solubilizing bacteria, Salinity, Suaeda nudiflora, 16SrDNA

Abstract

A study was carried out to isolate and characterize salt-tolerant beneficial bacteria from the soil collected from the rhizosphere of a halophytic plant (Suaeda nudiflora) from Gosaba, 24 Parganas (South), West Bengal, India located in the Sundarbans delta during January 2018. The physicochemical analysis of the soil showed that the soil was alkaline in nature with high salinity. The organic carbon, total nitrogen and available phosphorus status of the soil were low while the available potassium content was high. Bacterium N8 isolated from the rhizospheric soil possessed multiple growth-promoting activities i.e., the highest phosphate solubilizing efficiency (8.52 mg P g-1 of sugar), nitrogen-fixing capacity (0.7 mg of N g-1 of sugar), phosphatase enzyme activity and tolerance to salinity at 6% NaCl. A morphological study of this bacterium indicated that it was gram-positive, rod-shaped and was present in both single and chain form. The PCR amplification of genomic DNA (using 16S rDNA specific primers) showed a clear visible band at 1.5Kb. Sequencing of the amplicon followed by BLAST analysis depicted that the bacterium N8 exhibited the highest similarity (99%) with the genus Bacillus. The NCBI-Gen Bank accession number of the bacterium was SUB416213 N8 MH489431. The presence of members of the genus Bacillus in the rhizosphere soil of Suaeda nudiflora from Sundarbans is being reported for the first time. This study has led to a significant avenue for the use of the N8 bacterium as a potential salt-tolerant phosphate solubilizing bio-inoculum for improving nutrient availability in the coastal region.

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Author Biography

  • SUDIPTA TRIPATHI, Institute of Agricultural Science, University of Calcutta, Kolkata, West Bengal

    Present address: School of Environment and Disaster Management, RMVERI, Ramakrishna Mission Ashrama, Narendrapur, Kolkata – 700 103, West Bengal, India

References

Arora, S., Patel, P.N., Vanza, M.J. and Rao, G.G. (2014). Isolation and characterization of endophytic bacteria colonizing halophyte and other salt tolerant plant species from coastal Gujarat. African Journal of Microbiology Research 8(17): 1779-1788.

Asea, P.E.A., Kucey, R.M.N. and Stewart, J.W.B. (1988). Inorganic phosphate solubilization by two Penicillium species in solution culture and soil. Soil Biological Biochemic 20: 459-464. https://doi.org/10.1016/0038-0717(88)90058-2.

Bahadur, I., Maurya, B.R., Meena, V.S., Saha, M., Kumar, A. and Aeron, A. (2017). Mineral release dynamics of tricalcium phosphate and waste muscovite by mineral-solubilizing rhizobacteria isolated from Indo-Gangetic plain of India. Geomicrobiology Journal 34(5): 454-466.

Bandyopadhyay, B.K., Maji, B., Sen, H.S. and Tyagi, N.K. (2003). Coastal Soils of West Bengal – Their Nature, Distribution and Characteristics. Technical Bulletin No. 1/2003, Central Soil Salinity Research Institute, Regional Research Station, Canning Town, West Bengal, India. 62 p.

Bordeleau, L.M. and Prévost, D. (1994). Nodulation and nitrogen fixation in extreme environments. Plant and soil 161(1): 115-125.

Cappuccino, J.G. and Sherman, N. (2005). Microbiology: A Laboratory Manual, Pearson/Benjamin Cummings, San Francisco. 507 p.

Chatli, A.S., Beri, V. and Sidhu, B.S. (2008). Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh. Indian Journal of Microbiology 48: 267-273. https://doi.org/10.1007/s12088-008-0037-y.

Cherian, S. and Reddy, M.P. (2000). Salt tolerance in the halophyte Suaeda nudiflora Moq.: effect of NaCl on growth, ion accumulation and oxidative enzymes. Indian Journal of Plant Physiology 5(1): 32-37.

Farooqui, A., Srivastava, J. and Hussain, S.M. (2009). Comparative leaf epidermal morphology and foliar Na: K accumulation in Suaeda species: A case study from coastal ecosystem, India. Phytomorphology 59(3): 102-111.

Fernández, L., Agaras, B., Zalba, P., Wall, L.G. and Valverde, C. (2012). Pseudomonas spp. isolates with high phosphate-mobilizing potential and root colonization properties from agricultural bulk soils under no-till management. Biology and Fertility of Soils 48: 763-773. https://doi.org/10.1007/s00374-012-0665-6.

Goldstein, A.H. (1951). Future trends in research on microbial phosphate solubilization: one hundred years of insolubility. Ugeskr Laeger 113: 1618-1621.

Hanif, M.K., Hameed, S., Imran, A., Naqqash, T., Shahid, M. and Van Elsas, J.D. (2015). Isolation and characterization of a b-propeller gene containing phosphobacterium Bacillus subtilis strain KPS-11 for growth promotion of potato (Solanum tuberosum L.). Frontiers Microbiology 6: 583. https://doi.org/10.3389/fmicb.2015.00583.

Jackson, M.L. (1967). Soil Chemical Analysis, Prentice Hall of India Pvt. Ltd., New Delhi, India.

Jaiswal, B., Singh, S., Agrawal, S. B., Lokupitiya, E. and Agrawal, M. (2022). Improvements in Soil Physical, Chemical and Biological Properties at Natural Saline and Non-Saline Sites Under Different Management Practices. Environmental Management 69(5): 1005-1019.

Jha, A., Saxena, J. and Sharma, V. (2013). Investigation on phosphate solubilization potential of agricultural soil bacteria as affected by different phosphorus sources, temperature, salt, and pH. Communications in Soil Science and Plant Analysis 44(16): 2443-2458.

Ju, X.T., Xing, G.X., Chen, X.P., Zhang, S.L., Zhang, L.J., Liu, X.J. and Zhang, F.S. (2009). Reducing environmental risk by improving N management in intensive Chinese agricultural systems. Proceedings of the National Academy of Sciences 106(9): 3041-3046.

Kizilkaya, R. (2009). Nitrogen fixation capacity of Azotobacter spp. strains isolated from soils in different ecosystems and relationship between them and the microbiological properties of soils. Journal of Environmental Biology 30(1): 73-82.

Li, X., Luo, L., Yang, J., Li, B. and Yuan, H. (2015). Mechanisms for solubilization of various insoluble phosphates and activation of immobilized phosphates in different soils by an efficient and salinity-tolerant Aspergillus niger strain An2. Applied Biochemic Biotechnology 175: 2755-2768. https://doi.org/10.1007/s12010-014-1465-2.

Majeed, A., Abbasi, M.K., Hameed, S., Imran, A., and Rahim, N. (2015). Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Frontiers Microbiology 6:198. https://doi.org/10.3389/fmicb.2015.00198.

Makadia, M.O. and Panchal, N.S. (2016). Antimicrobial activity of endophytes isolated from Salvadora persica, Suaeda nudiflora and Cassia auriculata. European Journal of Biotechnology and Bioscience 4(8): 51-53.

Moore, E., Arnscheidt, A., Krüger, A., Strömpl, C. and Mau, M. (2004). Simplified protocols for the preparation of genomic DNA from bacterial cultures. Molecular Microbial Ecology Manual 1.01: 3-18.

Olsen, S.R. (1954). Estimation of Available Phosphorus in Soils by Extraction with Sodium Bicarbonate. USDA Circular No. 939, USDA, Washington, D.C. 19 p.

Oren, A. (2002). Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. Journal of Industrial Microbiology and Biotechnology 28: 56-63.

Osorio, N.W., and Habte, M. (2015). Effect of a phosphate-solubilizing fungus and an arbuscular mycorrhizal fungus on leucaena seedlings in tropical soils with contrasting phosphate sorption capacity. Plant Soil 389: 375-385. https://doi.org/10.1007/s11104-014-2357-5.

Paul, N.B., and Rao, W.S. (1971). Phosphate-dissolving bacteria in the rhizosphere of some cultivated legumes. Plant and Soil 35(1-3): 127-132.

Rietz, D.N. and Haynes, R.J. (2003). Effect of irrigation-induced salinity and sodicity on soil microbial activity. Soil Biology and Biochemistry 35: 845-854.

Sardinha, M., Muller, T., Schmeisky, H. and Joergensen, R.G. (2003). Microbial performance in soils along a salinity gradient under acidic conditions. Applied Soil Ecology 23: 237-244.

Sarkar, S., Gaydon, D.S., Brahmachari, K., Poulton, P.L., Chaki, A.K., Ray, K., Ghosh, A., Nanda, M.K. and Mainuddin, M. (2022). Testing APSIM in a complex saline coastal cropping environment. Environmental Modelling & Software 147: 105239.

Shahid, M., Hameed, S., Imran, A., Ali, S., and Van Elsas, J.D. (2012). Root colonization and growth promotion of sunflower (Helianthus annuus L.) by phosphate solubilizing Enterobacter sp. Fs-11. World Journal Microbiology Biotechnology 28: 2749-2758. https://doi.org/10.1007/s11274-012-1086-2.

Suraj Singh, R.K., Mazumdar, P.B., Bhattacharjee, M.K. and Sarma. J.D. (2013). Determination of nitrogen fixing capacity of bacteria isolated from the rhizosphere soil of Crotolaria pallida from the valley districts of Manipur, India. IOSR Journal of Pharmacy and Biological Sciences 8(4): 20-24.

Sylvia, D.M., Hartel, P.G., Furhmann, J. and Zuberer, D. (2005). Principles and Applications of Soil Microbiology. Second edition, Prentice Hall Inc., New Jersey, USA.

Tabatabi, M.A and Bremner, J.M. (1969). Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology Biochemistry 1: 301-307.

Tripathi, S., Chakraborty, K. and Bandyopadhyay, B.K. (2007). Enzyme activities and microbial biomass in coastal soils of India. Soils Biology Biochemistry 39: 2840-2848.

Tripathi, S., Kumari, S., Chakraborty, A., Gupta, A., Chakrabarti, K. and Bandyopadhyay, B.K. (2006). Microbial biomass and its activities in salt affected coastal soils. Biology and Fertility of Soils 42: 273-277.

Walkley, A. and Black, I.A. (1934). An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science 37(1): 29-38.

Wang, Z., Xu, G., Ma, P., Lin, Y., Yang, X. and Cao, C. (2017). Isolation and characterization of a phosphorus-solubilizing bacterium from rhizosphere soils and its colonization of chinese cabbage (Brassica campestris ssp. chinensis). Frontiers in Microbiology 8: 1270.

Xie, G.H., Cai, M.Y., Tao, G.C. and Steinberger, Y. (2003). Cultivable heterotrophic N2-fixing bacterial diversity in rice fields in the Yangtze River Plain. Biology and Fertility of Soils 37(1): 29-38.

Yadav, J.S.P., Bandyopadhyay, A.K. and Bandyopadhyay, B.K. (1983). Extent of coastal saline soil of India. Journal of Indian Society of Coastal Agriculture Research 1: 1-6.

Zhao, K., Penttinen, P., Zhang, X.P., Ao, X.L., Liu, M.K. and Yu, X.M. (2014). Maize rhizosphere in Sichuan, China, hosts plant growth promoting Burkholderia cepacia with phosphate solubilizing and antifungal abilities. Microbiology Research 169: 76-82. https://doi.org/10.1016/j.micres.2013.07.003.

Zhong, W.H., and Cai, Z.C. (2007). Long-term effects of inorganic fertilizers on microbial biomass and community functional diversity in a paddy soil derived from quaternary red clay. Applied Soil Ecology 36(2-3): 84-91.

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Submitted

2022-05-02

Published

2023-04-20

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BANERJEE, S., BANERJEE, A., MUKHERJEE, A. K., TRIPATHI, S., & CHATTERJEE, G. (2023). Isolation and Characterization of Beneficial Bacterium from the Rhizosphere of Suaeda nudiflora in Sundarbans, India. Journal of the Indian Society of Coastal Agricultural Research, 40(2), 48-57. https://doi.org/10.54894/JISCAR.40.2.2022.123565
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