nifH Composition Affecting Nitrogen Fixation in Soil: Study in Coastal Saline Region
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Authors
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S. BARUA
Department of Agricultural Chemistry and Soil Science. Institute of Agricultural Science University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal - 700 019
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B. DAS
Department of Agricultural Chemistry and Soil Science. Institute of Agricultural Science University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal - 700 019
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S. TRIPATHI
Department of Agricultural Chemistry and Soil Science. Institute of Agricultural Science University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal - 700 019
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K. CHAKRABARTI
Department of Agricultural Chemistry and Soil Science. Institute of Agricultural Science University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal - 700 019
Keywords:
Coastal soil, nifH, Soil DNA, PCR-RFLP, HaeIIIAbstract
The impact of change in salinity during different seasons in coastal saline soils of the Sundarbans, West Bengal (20° 15′N and 80° 40′E), India on PCR-RFLP of nifH gene pool was studied. PCR-RFLP of nifH gene pool was carried out by digestion of nifH amplicon from community DNA with HaeIII. Soils were collected during monsoon, winter and summer seasons of 2011-2012 from four different mono-cropped rice plots having variable salinity. Seasonal change in salinity was prominent and it detrimentally influenced the nifH gene pool both within the soil as well as the season. Temporal change in nifH composition maximally influenced dinitrogen fixation in soil as revealed by canonical correlation analysis (CCA). Dendogram generated from the RFLP pattern of nifH gene pool showed that community structure during monsoon was distinctly different from that of winter and summer. Pattern of the later two seasons belonged to the same cluster.
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