Evaluation of Plant Growth Promoting Potential and Nitrogen Fixing Efficiency of Halotolerant Azospirillum sp. Isolated from Coastal Paddy Fields


Abstract views: 82 / PDF downloads: 67

Authors

  • RANIT SARKAR Department of Microbiology, College of Basic Science and Humanities, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar - 751 003, Odisha, India
  • PRATIMA RAY Department of Microbiology, College of Basic Science and Humanities, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar - 751 003, Odisha, India
  • JOLLY TOPPO Department of Microbiology, College of Basic Science and Humanities, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar - 751 003, Odisha, India

https://doi.org/10.54894/JISCAR.41.2.2023.136629

Keywords:

Bioinoculants, Diazotrophs, Halotolerant, Plant growth-promoting attributes

Abstract

Salinity is a major abiotic constraint, affecting rice quality and productivity. Traditional methods and synthetic nitrogen fertilizers pose risks to the bio-economy and environment. Thus, the usage of halotolerant diazotrophic bioinoculants can be a sustainable approach for improving nitrogen nutrition and ameliorating saline stress in rice plants. On account of that, three Azospirillum sp. (AZS1, AZS2 & AZS3) were isolated from the rhizospheric soil of the coastal rice field in Odisha, India. Among them, AZS1 showed significantly high salt (8%) tolerance, various plant growth-promoting attributes (IAA, GA, HCN, ammonia production and inorganic P-solubilization), and extracellular enzymatic (amylase and cellulase) activities. Additionally, the isolate also showed elevated levels of superoxide dismutase (SOD) and proline production under increasing NaCl stress. This potential diazotroph (AZS1) was confirmed as Azospirillum sp. (OP503586) by 16S rRNA gene sequence. Further, the detection of the nifH gene and determination of the increasing percentage of nitrogen in culture media indicated the nitrogen-fixing ability of this isolate. In general, this study suggests the potential to utilize this indigenous halotolerant Azospirillum sp. as a potential bioinoculant to mitigate the salt stress in coastal agro-ecosystem. Nonetheless, a field study is highly essential in this regard prior to its practical application.

Downloads

Download data is not yet available.

References

Akhter, M.S., Hossain, S.J., Hossain, S.A. and Datta, R.K. (2012). Isolation and characterization of salinity tolerant Azotobacter sp. Greener Journal of Biological Science 2(3): 43-51.

Banik, A., Mukhopadhay, S.K. and Dangar, T.K. (2016). Characterization of N2-fixing plant growth promoting endophytic and epiphytic bacterial community of Indian cultivated and wild rice (Oryza sp.) genotypes. Planta 243: 799-812.

Bashan, Y. and De-Bashan, L.E. (2010). How the plant growth-promoting bacterium Azospirillum promotes plant growth - A critical assessment. Advances in Agronomy 108: 77-136.

Bates, L.S., Waldren, R.P. and Trare, L.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil 39: 205-207.

Bhavna, B., Shruti, S. and Sangeeta, S. (2019). Isolation and Characterization of halotolerant Azotobacter species from different coastal soils of South Gujarat Region. International Journal of Pharmacy and Biological Sciences 9(2): 774-779.

Chakraborty, K., Singh, A.L., Kalariya, K.A. and Goswami, N. (2015). Physiological responses of peanut (Arachis hypogaea L.) cultivars to water deficit stress: status of oxidative stress and antioxidant enzyme activities. Acta Botanica Croatica 74(1): 123-142.

Daliakopoulos, I.N., Tsanis, I.K., Koutroulis, A., Kourgialas, N.N., Varouchakis, A.E., Karatzas, G.P. and Ritsema, C.J. (2016). The threat of soil salinity: A European scale review. Science of the Total Environment 573: 727-739.

Dane, J.H. and Topp, C.G. (2020). Methods of Soil Analysis, Part 4: Physical Methods, John Wiley and Sons, NJ, USA. 1744 p.

El-Katatny, M.H.(2010). Enzyme production and nitrogen fixation by free, immobilized and coimmobilized inoculants of Trichoderma harzianum and Azospirillum brasilense and their possible role in growth promotion of tomato. Food Technology and Biotechnology 48(2): 161-174.

García, J.E., Maroniche, G., Creus, C., Suárez-Rodríguez, R., Ramirez-Trujillo, J.A. and Groppa, M.D. (2017). In vitro PGPR properties and osmotic tolerance of different Azospirillumnative strains and their effects on growth of maize under drought stress. Microbiological Research 202: 21-29.

Haiyambo, D.H. and Chimwamurombe, P.M. (2018). Isolation of the Azospirillum species from the rhizosphere of the leguminous Bauhinia petersiana in North Eastern Namibia. Jordan Journal of Biological Sciences 11(4): 347-353.

Holt, J.G., Krieg, N.R., Sneath, P.H.A., Stanley, J.T. and William, S.T. (1994). Bergey’s Manual of Determinative Bacteriology. Williams and Wilikins, Baltimore, USA. pp 786-788.

Hossain, M.M., Akter, S., Hasan, M.M., Hasan, A., Uddin, K.R., Parvin, A. and Rahman, S.B. (2014). Nitrogen fixing efficiency and physiological characteristics of Azospirillum isolates from the paddy fields of North Bengal. Jahangirnagar University Journal of Biological Sciences 3(1): 47-53.

Kazemeini, SA., Alinia, M. and Shakeri, E. (2016) Interaction effect of salinity stress and nitrogen on growth and activity of antioxidant enzymes of blue panicgrass (Panicum antidotale Retz.). Environmental Stresses in Crop Sciences 9(3): 279-289.

Khoma, Y.A., Nesterenko, O.G., Kutsokon, N.K., Khudolieieva, L.V., Shevchenko, V.V. and Rashydov, N.M. (2021). Proline content in the leaves of poplar and willow under water deficit. Regulatory Mechanisms in Biosystems 12(3): 519-522.

Krishnan, R., Menon, R.R., Tanaka, N., Busse, H-J., Krishnamurthi, S. and Rameshkumar, N. (2016). Arthrobacter pokkalii sp nov, a novel plant associated actinobacterium with plant beneficial properties, isolated from saline tolerant pokkali rice, Kerala, India. PLoS ONE 11(3): e0150322. https://doi.org/10.1371/journal.pone.0150322.

Lin, S., Liu, Y.C., Hameed, A., Hsu, Y.H., Huang, H.I., Lai, W.A. and Young, C.C. (2016). Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil. International Journal of Systematic and Evolutionary Microbiology 66(3):1453-1458.

Molina, R., López, G., Coniglio, A., Furlan, A., Mora, V., Rosas, S. and Cassán, F. (2021). Day and blue light modify growth, cell physiology and indole‐3‐acetic acid production of Azospirillum brasilense Az39 under planktonic growth conditions. Journal of Applied Microbiology 130(5): 1671-1683. https://doi.org/10.1111/jam.14869.

Nabti, E., Schmid, M. and Hartmann, A. (2015). Application of halotolerant bacteria to restore plant growth under salt stress. In: Halophiles - Biodiversity and Sustainable Exploitation, D.K. Maheshwari and M. Saraf (eds), Springer Cham. pp 235-259.

Narayan, R., Gupta, N.C. and Shahi, D.K. (2018). Isolation, morphological and cultural characterization of Azospirillum isolated from rhizospheric soils of various non-leguminous crops of Ranchi having acidic pH. International Journal of Current Microbiology and Applied Sciences 7(8): 329-338.

Narayanan, M., Pugazhendhi, A. and Ma, Y. (2022). Assessment of PGP traits of Bacillus cereus NDRMN001 and its influence on Cajanus cajan (L.) Millsp. phytoremediation potential on metal-polluted soil under controlled conditions. Frontiers in Plant Science 13: 1017043. https://doi.org/10.3389/fpls.2022.1017043.

Nawadkar, R.B., Jadhav, D.B. and Shaikh, N.R. (2015). Isolation of Azotobacter spp from saline soil and its applications on wheat (Tritium aestivum) plant for use in reclamation of saline soil with wheat plant. Research Journal of Life Sciences, Bioinformatics, Pharmaceutical and Chemical Sciences 1(1): 62-73.

Nina, G.C., Ana P. and Aharon, O. (2018). Strategies of adaptation of microorganisms of the three domains of life to high salt concentrations, FEMS Microbiology Reviews 42(3): 353-337.

Qadir, M., Quillérou, E., Nangia, V., Murtaza, G., Singh, M., Thomas, R.J. and Noble, A.D. (2014). Economics of salt‐induced land degradation and restoration. Natural Resources Forum 38(4): 282-295.

Richard, P.O., Adekanmbi, A.O. and Ogunjobi, A.A. (2018). Screening of bacteria isolated from the rhizosphere of maize plant (Zea mays L.) for ammonia production and nitrogen fixation. African Journal of Microbiology Research 12(34): 829-834.

Sulaiman, K.H., Al-Barakah, F.N., Assafed, A.M. and Dar, B.A.M. (2019). Isolation and identification of Azospirillum and Azotobacter species from Acacia spp. at Riyadh, Saudi Arabia. Bangladesh Journal of Botany 48(2): 239-251.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30(12): 2725-2729.

Tirry, N., Kouchou, A., Laghmari, G., Lemjereb, M., Hnadi, H., Amrani, K. and El Ghachtouli, N. (2021). Improved salinity tolerance of Medicago sativa and soil enzyme activities by PGPR. Biocatalysis and Agricultural Biotechnology 31: 101914. https://doi.org/10.1016/j.bcab.2021.101914.

Usha, D.K. and Kanimozhi, K. (2011). Isolation and characterization of saline tolerant Azospirillum strains from paddy field of Thanjavur district. Advances in Applied Science Research 2(3): 239-245.

Yasmeen, T., Ahmad, A., Arif, M.S., Mubin, M., Rehman, K., Shahzad, S.M., and Wijaya, L. (2020). Biofilm forming rhizobacteria enhance growth and salt tolerance in sunflower plants by stimulating antioxidant enzymes activity. Plant Physiology and Biochemistry 156: 242-256.

Zaied, K.A., El-Hadi, A.H.A., El-Shahawei, A.M. and Hamouda, A.S.M. (2009). Effect of azide resistant mutants induced in Azospirillum on wheat growth under salinity soil conditions. Research Journal of Agriculture Biological Science 5(6): 954-968.

Zarea, M.J., Hajinia, S., Karimi, N., Goltapeh, E.M., Rejali, F. and Varma, A. (2012). Effect of Piriformospora indica and Azospirillum strains from saline or non-saline soil on mitigation of the effects of NaCl. Soil Biology and Biochemistry 45: 139-146.

Zhang, T., Wang, T., Liu, K.S., Wang, L., Wang, K. and Zhou, Y. (2015). Effects of different amendments for the reclamation of coastal saline soil on soil nutrient dynamics and electrical conductivity responses. Agricultural Water Management 159: 115-122.

Downloads

Submitted

2023-05-20

Published

2024-01-09

Issue

Section

Articles

How to Cite

SARKAR, R. ., RAY, P., & TOPPO, J. . (2024). Evaluation of Plant Growth Promoting Potential and Nitrogen Fixing Efficiency of Halotolerant Azospirillum sp. Isolated from Coastal Paddy Fields. Journal of the Indian Society of Coastal Agricultural Research, 41(2), 15-27. https://doi.org/10.54894/JISCAR.41.2.2023.136629
Citation