Evaluation of genetic diversity of Deccani and Madgyal sheep from Southern peninsular region of India
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Authors
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R Arora
National Bureau of Animal Genetic Resources
Author
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S Bhatia
National Bureau of Animal Genetic Resources
Author
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B.P.Mishra
National Bureau of Animal Genetic Resources
Author
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B.Prakash
National Bureau of Animal Genetic Resources
Author
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H. K. Yadav
National Bureau of Animal Genetic Resources
Author
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A. Sahu
National Bureau of Animal Genetic Resources
Author
Keywords:
Deccani, Madgyal, Indian sheep, microsatellite, genetic diversity, genetic improvementAbstract
Information is presented on the genetic diversity analysis of Deccani and Madgyal, two peculiar sheep breeds of Maharashtra state of Southern peninsular zone of India, based on twenty five microsatellite markers recommended for ovines. All the markers were polymorphic in both the populations. Estimates of observed (na) and effective number of alleles (ne), allele diversity, gene diversity and fixation indices (f, è and F) were obtained. Genetic variation observed in terms of allele diversity (>7) and gene diversity (>0.6) revealed high level of genetic diversity within the investigated populations. There was no indication of a recent bottleneck in either population. A higher deficit of heterozygotes (FIS) was detected in Deccani (0.132) as compared to Madgyal (0.038). The estimation of è showed that the relative magnitude of population differentiation between the two populations was quite low at 1.4%. The Nei’s standard genetic distance between Deccani and Madgyal sheep was estimated to be 0.080. Considerably higher level of gene flow (14.3) observed between them was suggested to be enough to attenuate the genetic differentiation between the two sheep populations. A fairly lower degree of reliability was obtained for the individual breed assignment from the 25 loci using the Bayesian method with an accuracy of 85% only. The results showed that though there is high genetic diversity within each of Deccani and Madgyal sheep populations but there is a relatively low level of genetic differentiation between them. Negative value of relatedness reflected the influx of new germplasm in both the investigated populations. The results reported here would provide important information for the accomplishment of genetic improvement not only in the threatened Madgyal but also in Deccani-one of the numerically abundant breed from Maharashtra.
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