Evaluation of semen cryopreservation protocols for Kadaknath chicken
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Authors
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Shanmugam M
ICAR-Directorate of Poultry Research
Author
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Ram Krishna Mahapatra
Author
Keywords:
Chicken, Conservation, Cryopreservation, semen qualityAbstract
Kadaknath is a black pigmented chicken breed found in the central India. Ex situ in vitro conservation of chicken is mainly done by semen cryopreservation. Two independent experiments were conducted to evaluate semen cryopreservation protocols for Kadaknath chicken. Experiment 1- Kadaknath semen was cryopreserved using 8% Ethylene Glycol (EG) in Sasaki diluent (SD), Lake and Ravie diluent (LR) or Red Fowl Extender (RFE). Experiment 2- semen was cryopreserved using 8% Ethylene Glycol (EG) in SD, 4% dimethyl sulfoxide (DMSO) in SD or 4% DMSO in LR. Semen was cryopreserved in 0.5 ml plastic straws and were thawed at 5°C for 100 sec or 37°C for 30 sec for assessment. The pre and post-thaw semen was assessed for sperm motility, live and abnormal sperm and acrosome integrity. The post-thaw semen was inseminated in adult hens to study fertility. The post-thaw sperm motility, live sperm and acrosome intact sperm were significantly lower (P < 0.05) in cryopreserved semen in both experiments. In experiment 1, the maximum post-thaw fertility (8.8%) was obtained from the 8% EG SD cryopreserved semen. In experiment 2, the maximum post-taw fertility (14.5%) was obtained from the 4% DMSO SD cryopreserved semen. In conclusion, Kadaknath chicken semen may be cryopreserved using 4% DMSO in SD and thawed at 5°C for 100 sec for obtaining better fertility.
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