Differential expression of Pattern Recognition Receptor (PRR) genes in zebu (Bos indicus) and crossbred (Bos taurus x Bos indicus) cattle in response to Brucella abortus S19 antigen
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Authors
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D Ravikumar
Author
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Jayakumar S
Author
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Ranjit S Kataria
Author
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Saket Kumar Niranjan
ICAR-National Bureau of Animal Genetic Resources, Karnal
Author
Keywords:
Brucella abortus S19, Brucellosis, cattle, PRR Molecules , immunomodulationAbstract
Innate immunity is critical for the initial containment of the pathogen in the host; however, the strength may deviate in different host types. Here, we demonstrated the relative expression of pattern recognition receptor (PRR) molecules- Toll-like receptor (TLR) 4 and NOD-like receptor (NLR) 1 of immune cells, involved in the initial recognition of pathogens, during innate immunity in Bos indicus (zebu) and Bos taurus × Bos indicus (crossbred) genetic groups. Real-time expressions of TLR4 and NLR1 genes were evaluated in six female young calves of both groups before (0d) and after 7th, 14th and 28th day (d) immunization with attenuated Brucella abortus S19 strain. The receptor genes, in both of the groups showed downregulation during the initial stage (at 7d) of immunization, followed by upregulation at a later stage. The expression of the TLR4, a specific PRR to recognize gram-negative bacteria including B. abortus, was significantly (p<0.01) higher in crossbred than that of zebu at 14d and 28d. However, the expression of the NLR1, a generalized PRR, was significant (p<0.05) higher initially (14d) in crossbred, but later (28d) in zebu. The differential expression of PRR molecules observed in both kinds of cattle groups indicated their diverse pattern and strength of containment of the Brucella organism at an initial stage.
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