Effect of Foliar Application of Salicylic Acid on Enhancing Salinity Tolerance in Sorghum (Sorghum bicolor L.)
Foliar application of salicylic acid for enhancing salinity tolerance
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Keywords:
Anti–oxidant activity, Carotenoids, Chlorophyll, Proline, Salinity, Salicylic acidAbstract
Salinity represents a major abiotic constraint that adversely affects crop productivity, particularly in arid and semi-arid regions. Under saline conditions, plant growth and development are primarily hindered by physiological drought, ionic toxicity resulting from excessive accumulation of sodium (Na+) and chloride (Cl-) ions, and the generation of reactive oxygen species (ROS) such as superoxide radicals, hydrogen peroxide, hydroxyl ions, and singlet oxygen within chloroplasts, mitochondria, and the apoplastic space. To address the impact of salinity stress, a field experiment was conducted during the Kharif seasons of 2023, 2024, and 2025 at a farmer’s field in Kakarlamoodi village. The objective was to evaluate the efficacy of foliar application of salicylic acid in enhancing salinity tolerance in sorghum. The study was structured using a randomized block design comprising six treatments, each replicated four times. Five concentrations of salicylic acid (50, 100, 150, 200, and 250 mg L-¹) were applied, along with a control treatment. The results demonstrated that the 250 mg L-¹ salicylic acid treatment significantly improved grain yield (5729, 6235 and 6300 kg ha-¹), whereas the lowest yield was recorded under the control treatment. In addition to yield enhancement, the highest values for total chlorophyll content, antioxidant enzyme activity, protein concentration, and potassium accumulation in both grain and straw were observed with the 250 mg L-1 foliar application, indicating its effectiveness in mitigating the adverse effects of salinity.
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Amini F and Ehsanpour AA (2005) Soluble proteins, proline, carbohydrates and Na+/K+ changes in two tomato (Lycopersicon esculentum Mill.) cultivars under In vitro salt stress. American Journal of Biochemistry and Biotechnology 1(4): 204-208.
Chai YY, Jiang CD, Shi L, Shi TS, Gu WB (2010) Effects of exogenous spermine on sweet sorghum during germination under salinity. Biologia Plantarum 54:145–148.
Dehnavi AR, Zahedi M, Ludwiczak A, Piernik, A (2022) Foliar application of salicylic acid improves salt tolerance of sorghum (Sorghum bicolor (L.) Moench). Plants 11(3): 368.
Durner J, Klessig DF (1996) Salicylic acid is a modulator of tobacco and mammalian catalases. Journal of Biological Chemistry 271: 28492–28501.
El-Tayeb MA (2005) Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation 45: 215-224.
Gupta B, Huang B (2014) Mechanism of salinity tolerance in plants: Physiological, biochemical, and molecular characterization. International Journal of Genomics 2014(1): 701596.
Hanif S, Mahmood A, Javed T, Bibi S, Zia MA, Asghar S, Naeem Z, Ercisli S, Rahimi M, Ali B (2024) Exogenous application of salicylic acid ameliorates salinity stress in barley (Hordeum vulgare L.). BMC Plant Biology 24(1): 270.
Jaiswal A, Pandurangam V, Sharma SK (2014) Effect of salicylic acid in soybean (Glycine max L. Meril) under salinity stress. The Bioscan 9(2): 671-676.
Jangra M, Devi S, Satpal, Kumar N, Goyal V, Mehrotra S (2023) Amelioration effect of salicylic acid under salt stress in Sorghum bicolor L. Applied Biochemistry and Biotechnology 194(10): 4400–4423.
Leegood RC (2006) Handbook of photosynthesis. In Annals of Botany, 2nd ed.; Pessarakli, M., Ed.; CRC Press: Boca Raton, FL, USA 97:152–153.
Mundada PS, Jadhav SV, Salunkhe SS, Gurme ST, Umdale SD, Nikam TD, Ahire ML (2021) Plant Performance and Defensive Role of Proline Under Environmental Stress. In Plant Performance Under Environmental Stress. Springer 201–223.
Noreen S, Shakeela N, Ahmad S, Fehmeeda B, Hasanuzzaman M (2016) Quantifying some physiological and productivity indices of canola (Brassica napus L.) crop under an arid environment. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44: 272–279.
Pan T, Liu M, Kreslavski VD, Zharmukhamedov SK, Nie C, Kuznetsov VV, Allakhverdiev SI, Shabala S (2021) Non-stomatal limitation of photosynthesis by soil salinity. Critical Reviews in Environmental Science and Technology 51: 791–825.
Panse VG, Sukhatme VG (1978) Statistical Methods for Agricultural Workers, 2nd Edition. Indian Council of Agricultural Research.
Quamruzzaman M, Manik S, Shabala S, Zhou M (2021) Improving Performance of Salt-Grown Crops by Exogenous Application of Plant Growth Regulators. Biomolecules 11:788.
Saleem M, Fariduddin Q, Castroverde CDM (2021) Salicylic acid: A key regulator of redox signalling and plant immunity. Plant Physiology and Biochemistry 168 :381–397.
Shrivastava P and Kumar R (2015) Soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences 22: 123–131.
Singh M, Kumar J, Singh S, Singh VP, Prasad SM (2015) Roles of osmoprotectants in improving salinity and drought tolerance in plants: A review. Reviews in Environmental Science and Biotechnology 14: 407–426.
Singh NB, Khare S, Singh A, Yadav V, Yadav RK (2021) Salicylic acid and Indole acetic acid synergistically ameliorates Ferulic acid toxicity in Brassica juncea L. seedlings. Plant Physiology Reports 26: 729–740.
Sofy MR, Elhawat N, Alshaal T (2020) Glycine betaine counters salinity stress by maintaining high K+/Na+ ratio and antioxidant defense via limiting Na+ uptake in common bean (Phaseolus vulgaris L). Ecotoxicological and Environmental Safety 200: 110732.
Spychalla JP, Desborough SL (1990) Superoxide dismutase, catalase, and α-tocopherol content of stored potato tubers. Plant Physiology 94: 1214–1218.
Sytar O, Brestic M, Zivcak M, Olsovska K, Kovar M,. Shao H, He X (2017) Applying hyperspectral imaging to explore natural plant diversity towards improving salt stress tolerance. Science of the Total Environment 578: 90–99.
Turner NC (2018) Turgor maintenance by osmotic adjustment: 40 years of progress. Journal of Experimental Botany 69: 3223–3233.
Verbruggen N, Hermans C (2008) Proline accumulation in plants: A review. Amino Acids 35: 753–759.
Yu Z, Duan X, Luo L, Dai S, Ding Z, Xia G (2020) How plant hormones mediate salt stress responses. Trends in Plant Sciences 25: 1117–1130.
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Journal of Soil Salinity and Water Quality serves as an official organ of the Indian Society of Soil Salinity and Water Quality (ISSSWQ) for the publication of research papers, reviews, and short communications as per the constitution and by-laws of the society. Soft and hard copy of the journal are sent free to all its members. All disputes are subject to the exclusive jurisdiction of competent court and forums in Kamal only. The society does not assume any responsibility for opinion by the authors in the articles and no-material in any form can be reproduced without the prior permission of the society. The society is not responsible for any delay, whatsoever, in publication/ delivery of the periodicals to the subscribers due to unforeseen circumstances or postal delay. The society does not vouch for any claims made by the advertisers of products and services. The publisher and the editors shall not be held liable for any consequences in the event of such claim not being honoured by the advertisers.