Microsatellite Markers-Based Genetic Diversity and Population Structure Analysis of Lentil (Lens culinaris Medik.) Accessions
Microsatellite markers-based genetic diversity of lentil
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Authors
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Pooja Kanwar Shekhawat
Sardarkrushinagar Dantiwada Agricultural University, Banaskantha, Sardarkrushinagar – 385506, Gujarat, India
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Vivek Singh
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
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Ankit Kumar
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
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Zeetendra Singh
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
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Jogendra Singh
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
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Vijayata Singh
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
Keywords:
Lens culinaris, Germplasm, Genetic diversity, SSR markers, Population structureAbstract
Lentil is a nutritious, protein-rich winter-season pulse crop acting as a boon for mitigating malnutrition and maintaining global food security. Genetic variation and selection is the basis for any crop improvement programme. To explore the pattern of genetic diversity among lentil accessions under salt stress, microsatellite (SSR) markers were used. The development of long-lasting, stable cultivars requires the utilisation of diverse germplasm. This study was conducted at the ICAR-Central Soil Salinity Research Institute, Karnal, using 100 lentil accessions. In this study, 12 SSR (simple sequence repeat) markers were used. Minor allele frequency (MAF) ranged from 0.07 to 0.49, with a mean value of 0.27. Genetic diversity (GD) for SSRs ranged from 0.11 to 0.50. The polymorphic information content (PIC), which ranged from 0 to 1, was 0.12 to 0.38 in the present study. Three sub-groups based on population structure analysis, denoted as the red, green and blue clusters, encompassed of 35, 48 and 17 lentil accessions, respectively. A UPGMA-based phylogenetic tree was also constructed, which grouped all genotypes into three main clusters. The information generated in our study may have significant implications for molecular characterisation, fingerprinting, and documentation of accessions in the lentil improvement programme.
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